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Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery. Electrotransfer traditionally relies upon the application of direct current pulses...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584139/ https://www.ncbi.nlm.nih.gov/pubmed/37851653 http://dx.doi.org/10.1371/journal.pone.0293035 |
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author | Skinner, Molly A. Otten, Alex Hoff, Andrew Jaroszeski, Mark |
author_facet | Skinner, Molly A. Otten, Alex Hoff, Andrew Jaroszeski, Mark |
author_sort | Skinner, Molly A. |
collection | PubMed |
description | With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery. Electrotransfer traditionally relies upon the application of direct current pulses to the cells of interest. Corona charge was investigated in this study as an alternative to traditional methods as a means of creating the electric field necessary to deliver materials via electrotransfer. The goal was to determine if there was an increase in molecular delivery across the membrane of a human T cell line used as a model system. In a novel dish created for the study, the effects of elevated temperatures (37, 40, 43, and 45°C) during the treatment process were also examined in combination with corona charge application. Results showed that treating cells with corona charge at room temperature (~23°C) caused a statistically significant increase in molecular delivery while maintaining viability. Heat alone did not cause a statistically significant effect on molecular delivery. Combined corona charge treatment and heating resulted in a statistically significant increase on molecular delivery compared to controls that were only heated. Combined corona charge treatment and heating to all temperatures when compared to controls treated at room temperature, showed a statistically significant increase in molecular delivery. |
format | Online Article Text |
id | pubmed-10584139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105841392023-10-19 Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro Skinner, Molly A. Otten, Alex Hoff, Andrew Jaroszeski, Mark PLoS One Research Article With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery. Electrotransfer traditionally relies upon the application of direct current pulses to the cells of interest. Corona charge was investigated in this study as an alternative to traditional methods as a means of creating the electric field necessary to deliver materials via electrotransfer. The goal was to determine if there was an increase in molecular delivery across the membrane of a human T cell line used as a model system. In a novel dish created for the study, the effects of elevated temperatures (37, 40, 43, and 45°C) during the treatment process were also examined in combination with corona charge application. Results showed that treating cells with corona charge at room temperature (~23°C) caused a statistically significant increase in molecular delivery while maintaining viability. Heat alone did not cause a statistically significant effect on molecular delivery. Combined corona charge treatment and heating resulted in a statistically significant increase on molecular delivery compared to controls that were only heated. Combined corona charge treatment and heating to all temperatures when compared to controls treated at room temperature, showed a statistically significant increase in molecular delivery. Public Library of Science 2023-10-18 /pmc/articles/PMC10584139/ /pubmed/37851653 http://dx.doi.org/10.1371/journal.pone.0293035 Text en © 2023 Skinner et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Skinner, Molly A. Otten, Alex Hoff, Andrew Jaroszeski, Mark Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro |
title | Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro |
title_full | Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro |
title_fullStr | Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro |
title_full_unstemmed | Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro |
title_short | Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro |
title_sort | combined effect of heat and corona charge on molecular delivery to a t cell line in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584139/ https://www.ncbi.nlm.nih.gov/pubmed/37851653 http://dx.doi.org/10.1371/journal.pone.0293035 |
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