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Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions

Large animal models are frequently used to investigate new medical approaches. In most cases, animals are kept under general anesthesia and mandatory mechanical ventilation during the experiments. However, in some situations assisted spontaneous breathing is essential, e.g. when simulating condition...

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Autores principales: Wenzel, Christin, Spassov, Sashko G., Haberstroh, Jörg, Spaeth, Johannes, Schumann, Stefan, Schmidt, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584169/
https://www.ncbi.nlm.nih.gov/pubmed/37851695
http://dx.doi.org/10.1371/journal.pone.0293215
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author Wenzel, Christin
Spassov, Sashko G.
Haberstroh, Jörg
Spaeth, Johannes
Schumann, Stefan
Schmidt, Johannes
author_facet Wenzel, Christin
Spassov, Sashko G.
Haberstroh, Jörg
Spaeth, Johannes
Schumann, Stefan
Schmidt, Johannes
author_sort Wenzel, Christin
collection PubMed
description Large animal models are frequently used to investigate new medical approaches. In most cases, animals are kept under general anesthesia and mandatory mechanical ventilation during the experiments. However, in some situations assisted spontaneous breathing is essential, e.g. when simulating conditions in a modern intensive care unit. Therefore, we established an anesthesia regime with dexmedetomidine and midazolam/ketamine in porcine models of assisted spontaneous breathing. The total intravenous anesthesia was used in lung healthy pigs, in pigs with oleic acid induced acute respiratory distress syndrome and in pigs with methacholine induced bronchopulmonary obstruction. We were able to maintain stable conditions of assisted spontaneous breathing without impairment of hemodynamic, respiratory or blood gas variables in lung healthy pigs and pigs with induced acute respiratory distress syndrome for a period of five hours and in pigs with induced bronchopulmonary obstruction for three hours. Total intravenous anesthesia containing dexmedetomidine enables stable conditions of assisted spontaneous breathing in healthy pigs, in pigs with induced acute respiratory distress syndrome and in pigs induced bronchopulmonary obstruction as models of intensive care unit conditions.
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spelling pubmed-105841692023-10-19 Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions Wenzel, Christin Spassov, Sashko G. Haberstroh, Jörg Spaeth, Johannes Schumann, Stefan Schmidt, Johannes PLoS One Research Article Large animal models are frequently used to investigate new medical approaches. In most cases, animals are kept under general anesthesia and mandatory mechanical ventilation during the experiments. However, in some situations assisted spontaneous breathing is essential, e.g. when simulating conditions in a modern intensive care unit. Therefore, we established an anesthesia regime with dexmedetomidine and midazolam/ketamine in porcine models of assisted spontaneous breathing. The total intravenous anesthesia was used in lung healthy pigs, in pigs with oleic acid induced acute respiratory distress syndrome and in pigs with methacholine induced bronchopulmonary obstruction. We were able to maintain stable conditions of assisted spontaneous breathing without impairment of hemodynamic, respiratory or blood gas variables in lung healthy pigs and pigs with induced acute respiratory distress syndrome for a period of five hours and in pigs with induced bronchopulmonary obstruction for three hours. Total intravenous anesthesia containing dexmedetomidine enables stable conditions of assisted spontaneous breathing in healthy pigs, in pigs with induced acute respiratory distress syndrome and in pigs induced bronchopulmonary obstruction as models of intensive care unit conditions. Public Library of Science 2023-10-18 /pmc/articles/PMC10584169/ /pubmed/37851695 http://dx.doi.org/10.1371/journal.pone.0293215 Text en © 2023 Wenzel et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wenzel, Christin
Spassov, Sashko G.
Haberstroh, Jörg
Spaeth, Johannes
Schumann, Stefan
Schmidt, Johannes
Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions
title Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions
title_full Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions
title_fullStr Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions
title_full_unstemmed Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions
title_short Establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: A preclinical model of intensive care conditions
title_sort establishment and validation of intravenous anesthesia with dexmedetomidine for pigs under assisted spontaneous breathing: a preclinical model of intensive care conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584169/
https://www.ncbi.nlm.nih.gov/pubmed/37851695
http://dx.doi.org/10.1371/journal.pone.0293215
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