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SIRT-1 is required for release of enveloped enteroviruses
Enterovirus D68 (EV-D68) is a re-emerging enterovirus that causes acute respiratory illness in infants and has recently been linked to Acute Flaccid Myelitis. Here, we show that the histone deacetylase, SIRT-1, is essential for autophagy and EV-D68 infection. Knockdown of SIRT-1 inhibits autophagy a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584371/ https://www.ncbi.nlm.nih.gov/pubmed/37850626 http://dx.doi.org/10.7554/eLife.87993 |
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author | Jassey, Alagie Logue, James Weston, Stuart Wagner, Michael A Galitska, Ganna Miller, Katelyn Frieman, Matthew Jackson, William T |
author_facet | Jassey, Alagie Logue, James Weston, Stuart Wagner, Michael A Galitska, Ganna Miller, Katelyn Frieman, Matthew Jackson, William T |
author_sort | Jassey, Alagie |
collection | PubMed |
description | Enterovirus D68 (EV-D68) is a re-emerging enterovirus that causes acute respiratory illness in infants and has recently been linked to Acute Flaccid Myelitis. Here, we show that the histone deacetylase, SIRT-1, is essential for autophagy and EV-D68 infection. Knockdown of SIRT-1 inhibits autophagy and reduces EV-D68 extracellular titers. The proviral activity of SIRT-1 does not require its deacetylase activity or functional autophagy. SIRT-1’s proviral activity is, we demonstrate, mediated through the repression of endoplasmic reticulum stress (ER stress). Inducing ER stress through thapsigargin treatment or SERCA2A knockdown in SIRT-1 knockdown cells had no additional effect on EV-D68 extracellular titers. Knockdown of SIRT-1 also decreases poliovirus and SARS-CoV-2 titers but not coxsackievirus B3. In non-lytic conditions, EV-D68 is primarily released in an enveloped form, and SIRT-1 is required for this process. Our data show that SIRT-1, through its translocation to the cytosol, is critical to promote the release of enveloped EV-D68 viral particles. |
format | Online Article Text |
id | pubmed-10584371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-105843712023-10-19 SIRT-1 is required for release of enveloped enteroviruses Jassey, Alagie Logue, James Weston, Stuart Wagner, Michael A Galitska, Ganna Miller, Katelyn Frieman, Matthew Jackson, William T eLife Cell Biology Enterovirus D68 (EV-D68) is a re-emerging enterovirus that causes acute respiratory illness in infants and has recently been linked to Acute Flaccid Myelitis. Here, we show that the histone deacetylase, SIRT-1, is essential for autophagy and EV-D68 infection. Knockdown of SIRT-1 inhibits autophagy and reduces EV-D68 extracellular titers. The proviral activity of SIRT-1 does not require its deacetylase activity or functional autophagy. SIRT-1’s proviral activity is, we demonstrate, mediated through the repression of endoplasmic reticulum stress (ER stress). Inducing ER stress through thapsigargin treatment or SERCA2A knockdown in SIRT-1 knockdown cells had no additional effect on EV-D68 extracellular titers. Knockdown of SIRT-1 also decreases poliovirus and SARS-CoV-2 titers but not coxsackievirus B3. In non-lytic conditions, EV-D68 is primarily released in an enveloped form, and SIRT-1 is required for this process. Our data show that SIRT-1, through its translocation to the cytosol, is critical to promote the release of enveloped EV-D68 viral particles. eLife Sciences Publications, Ltd 2023-10-18 /pmc/articles/PMC10584371/ /pubmed/37850626 http://dx.doi.org/10.7554/eLife.87993 Text en © 2023, Jassey et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Jassey, Alagie Logue, James Weston, Stuart Wagner, Michael A Galitska, Ganna Miller, Katelyn Frieman, Matthew Jackson, William T SIRT-1 is required for release of enveloped enteroviruses |
title | SIRT-1 is required for release of enveloped enteroviruses |
title_full | SIRT-1 is required for release of enveloped enteroviruses |
title_fullStr | SIRT-1 is required for release of enveloped enteroviruses |
title_full_unstemmed | SIRT-1 is required for release of enveloped enteroviruses |
title_short | SIRT-1 is required for release of enveloped enteroviruses |
title_sort | sirt-1 is required for release of enveloped enteroviruses |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584371/ https://www.ncbi.nlm.nih.gov/pubmed/37850626 http://dx.doi.org/10.7554/eLife.87993 |
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