Cargando…

SIRT-1 is required for release of enveloped enteroviruses

Enterovirus D68 (EV-D68) is a re-emerging enterovirus that causes acute respiratory illness in infants and has recently been linked to Acute Flaccid Myelitis. Here, we show that the histone deacetylase, SIRT-1, is essential for autophagy and EV-D68 infection. Knockdown of SIRT-1 inhibits autophagy a...

Descripción completa

Detalles Bibliográficos
Autores principales: Jassey, Alagie, Logue, James, Weston, Stuart, Wagner, Michael A, Galitska, Ganna, Miller, Katelyn, Frieman, Matthew, Jackson, William T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584371/
https://www.ncbi.nlm.nih.gov/pubmed/37850626
http://dx.doi.org/10.7554/eLife.87993
_version_ 1785122724475043840
author Jassey, Alagie
Logue, James
Weston, Stuart
Wagner, Michael A
Galitska, Ganna
Miller, Katelyn
Frieman, Matthew
Jackson, William T
author_facet Jassey, Alagie
Logue, James
Weston, Stuart
Wagner, Michael A
Galitska, Ganna
Miller, Katelyn
Frieman, Matthew
Jackson, William T
author_sort Jassey, Alagie
collection PubMed
description Enterovirus D68 (EV-D68) is a re-emerging enterovirus that causes acute respiratory illness in infants and has recently been linked to Acute Flaccid Myelitis. Here, we show that the histone deacetylase, SIRT-1, is essential for autophagy and EV-D68 infection. Knockdown of SIRT-1 inhibits autophagy and reduces EV-D68 extracellular titers. The proviral activity of SIRT-1 does not require its deacetylase activity or functional autophagy. SIRT-1’s proviral activity is, we demonstrate, mediated through the repression of endoplasmic reticulum stress (ER stress). Inducing ER stress through thapsigargin treatment or SERCA2A knockdown in SIRT-1 knockdown cells had no additional effect on EV-D68 extracellular titers. Knockdown of SIRT-1 also decreases poliovirus and SARS-CoV-2 titers but not coxsackievirus B3. In non-lytic conditions, EV-D68 is primarily released in an enveloped form, and SIRT-1 is required for this process. Our data show that SIRT-1, through its translocation to the cytosol, is critical to promote the release of enveloped EV-D68 viral particles.
format Online
Article
Text
id pubmed-10584371
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-105843712023-10-19 SIRT-1 is required for release of enveloped enteroviruses Jassey, Alagie Logue, James Weston, Stuart Wagner, Michael A Galitska, Ganna Miller, Katelyn Frieman, Matthew Jackson, William T eLife Cell Biology Enterovirus D68 (EV-D68) is a re-emerging enterovirus that causes acute respiratory illness in infants and has recently been linked to Acute Flaccid Myelitis. Here, we show that the histone deacetylase, SIRT-1, is essential for autophagy and EV-D68 infection. Knockdown of SIRT-1 inhibits autophagy and reduces EV-D68 extracellular titers. The proviral activity of SIRT-1 does not require its deacetylase activity or functional autophagy. SIRT-1’s proviral activity is, we demonstrate, mediated through the repression of endoplasmic reticulum stress (ER stress). Inducing ER stress through thapsigargin treatment or SERCA2A knockdown in SIRT-1 knockdown cells had no additional effect on EV-D68 extracellular titers. Knockdown of SIRT-1 also decreases poliovirus and SARS-CoV-2 titers but not coxsackievirus B3. In non-lytic conditions, EV-D68 is primarily released in an enveloped form, and SIRT-1 is required for this process. Our data show that SIRT-1, through its translocation to the cytosol, is critical to promote the release of enveloped EV-D68 viral particles. eLife Sciences Publications, Ltd 2023-10-18 /pmc/articles/PMC10584371/ /pubmed/37850626 http://dx.doi.org/10.7554/eLife.87993 Text en © 2023, Jassey et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Jassey, Alagie
Logue, James
Weston, Stuart
Wagner, Michael A
Galitska, Ganna
Miller, Katelyn
Frieman, Matthew
Jackson, William T
SIRT-1 is required for release of enveloped enteroviruses
title SIRT-1 is required for release of enveloped enteroviruses
title_full SIRT-1 is required for release of enveloped enteroviruses
title_fullStr SIRT-1 is required for release of enveloped enteroviruses
title_full_unstemmed SIRT-1 is required for release of enveloped enteroviruses
title_short SIRT-1 is required for release of enveloped enteroviruses
title_sort sirt-1 is required for release of enveloped enteroviruses
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584371/
https://www.ncbi.nlm.nih.gov/pubmed/37850626
http://dx.doi.org/10.7554/eLife.87993
work_keys_str_mv AT jasseyalagie sirt1isrequiredforreleaseofenvelopedenteroviruses
AT loguejames sirt1isrequiredforreleaseofenvelopedenteroviruses
AT westonstuart sirt1isrequiredforreleaseofenvelopedenteroviruses
AT wagnermichaela sirt1isrequiredforreleaseofenvelopedenteroviruses
AT galitskaganna sirt1isrequiredforreleaseofenvelopedenteroviruses
AT millerkatelyn sirt1isrequiredforreleaseofenvelopedenteroviruses
AT friemanmatthew sirt1isrequiredforreleaseofenvelopedenteroviruses
AT jacksonwilliamt sirt1isrequiredforreleaseofenvelopedenteroviruses