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Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing
Non-cleaving Cas9 (dCas9) is widely employed to manipulate specific gene loci, often with scant regard for unintended transcriptional effects. We demonstrate here that dCas9 mediates precise RNA polymerase II transcriptional pausing followed by transcription termination and potential alternative pol...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584677/ https://www.ncbi.nlm.nih.gov/pubmed/37783853 http://dx.doi.org/10.1038/s41594-023-01090-9 |