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Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice

INTRODUCTION: Influenza vaccines play a vital role in protecting individuals from influenza virus infection and severe illness. However, current influenza vaccines have suboptimal efficacy, which is further reduced in cases where the vaccine strains do not match the circulating strains. One strategy...

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Autores principales: Beukema, Martin, Gong, Shuran, Al-Jaawni, Kasem, de Vries-Idema, Jacqueline J., Krammer, Florian, Zhou, Fan, Cox, Rebecca Jane, Huckriede, Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585035/
https://www.ncbi.nlm.nih.gov/pubmed/37869002
http://dx.doi.org/10.3389/fimmu.2023.1249902
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author Beukema, Martin
Gong, Shuran
Al-Jaawni, Kasem
de Vries-Idema, Jacqueline J.
Krammer, Florian
Zhou, Fan
Cox, Rebecca Jane
Huckriede, Anke
author_facet Beukema, Martin
Gong, Shuran
Al-Jaawni, Kasem
de Vries-Idema, Jacqueline J.
Krammer, Florian
Zhou, Fan
Cox, Rebecca Jane
Huckriede, Anke
author_sort Beukema, Martin
collection PubMed
description INTRODUCTION: Influenza vaccines play a vital role in protecting individuals from influenza virus infection and severe illness. However, current influenza vaccines have suboptimal efficacy, which is further reduced in cases where the vaccine strains do not match the circulating strains. One strategy to enhance the efficacy of influenza vaccines is by extended antigen delivery, thereby mimicking the antigen kinetics of a natural infection. Prolonging antigen availability was shown to quantitatively enhance influenza virus-specific immune responses but how it affects the quality of the induced immune response is unknown. Therefore, the current study aimed to investigate whether prolongation of the delivery of influenza vaccine improves the quality of the induced immune responses over that induced by prime-boost immunization. METHODS: Mice were given daily doses of whole inactivated influenza virus vaccine for periods of 14, 21, or 28 days; the control group received prime-boost immunization with a 28 days interval. RESULTS: Our data show that the highest levels of cellular and humoral immune responses were induced by 28 days of extended antigen delivery, followed by 21, and 14 days of delivery, and prime-boost immunization. Moreover, prolonging vaccine delivery also improved the quality of the induced antibody response, as indicated by higher level of high avidity antibodies, a balanced IgG subclass profile, and a higher level of cross-reactive antibodies. CONCLUSIONS: Our findings contribute to a better understanding of the immune response to influenza vaccination and have important implications for the design and development of future slow-release influenza vaccines.
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spelling pubmed-105850352023-10-20 Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice Beukema, Martin Gong, Shuran Al-Jaawni, Kasem de Vries-Idema, Jacqueline J. Krammer, Florian Zhou, Fan Cox, Rebecca Jane Huckriede, Anke Front Immunol Immunology INTRODUCTION: Influenza vaccines play a vital role in protecting individuals from influenza virus infection and severe illness. However, current influenza vaccines have suboptimal efficacy, which is further reduced in cases where the vaccine strains do not match the circulating strains. One strategy to enhance the efficacy of influenza vaccines is by extended antigen delivery, thereby mimicking the antigen kinetics of a natural infection. Prolonging antigen availability was shown to quantitatively enhance influenza virus-specific immune responses but how it affects the quality of the induced immune response is unknown. Therefore, the current study aimed to investigate whether prolongation of the delivery of influenza vaccine improves the quality of the induced immune responses over that induced by prime-boost immunization. METHODS: Mice were given daily doses of whole inactivated influenza virus vaccine for periods of 14, 21, or 28 days; the control group received prime-boost immunization with a 28 days interval. RESULTS: Our data show that the highest levels of cellular and humoral immune responses were induced by 28 days of extended antigen delivery, followed by 21, and 14 days of delivery, and prime-boost immunization. Moreover, prolonging vaccine delivery also improved the quality of the induced antibody response, as indicated by higher level of high avidity antibodies, a balanced IgG subclass profile, and a higher level of cross-reactive antibodies. CONCLUSIONS: Our findings contribute to a better understanding of the immune response to influenza vaccination and have important implications for the design and development of future slow-release influenza vaccines. Frontiers Media S.A. 2023-10-05 /pmc/articles/PMC10585035/ /pubmed/37869002 http://dx.doi.org/10.3389/fimmu.2023.1249902 Text en Copyright © 2023 Beukema, Gong, Al-Jaawni, de Vries-Idema, Krammer, Zhou, Cox and Huckriede https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Beukema, Martin
Gong, Shuran
Al-Jaawni, Kasem
de Vries-Idema, Jacqueline J.
Krammer, Florian
Zhou, Fan
Cox, Rebecca Jane
Huckriede, Anke
Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
title Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
title_full Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
title_fullStr Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
title_full_unstemmed Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
title_short Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
title_sort prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585035/
https://www.ncbi.nlm.nih.gov/pubmed/37869002
http://dx.doi.org/10.3389/fimmu.2023.1249902
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