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Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash

The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study describes the isolation and characterization of a novel Salmonella Typhimurium phage ‘Arash’ from hospital wastewater in Leuven, Belgium. Arash has a myovirus...

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Autores principales: Yousefi, Mohammad Hashem, Wagemans, Jeroen, Shekarforoush, Seyed Shahram, Vallino, Marta, Pozhydaieva, Nadiia, Höfer, Katharina, Lavigne, Rob, Hosseinzadeh, Saeid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585845/
https://www.ncbi.nlm.nih.gov/pubmed/37858092
http://dx.doi.org/10.1186/s12866-023-03056-9
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author Yousefi, Mohammad Hashem
Wagemans, Jeroen
Shekarforoush, Seyed Shahram
Vallino, Marta
Pozhydaieva, Nadiia
Höfer, Katharina
Lavigne, Rob
Hosseinzadeh, Saeid
author_facet Yousefi, Mohammad Hashem
Wagemans, Jeroen
Shekarforoush, Seyed Shahram
Vallino, Marta
Pozhydaieva, Nadiia
Höfer, Katharina
Lavigne, Rob
Hosseinzadeh, Saeid
author_sort Yousefi, Mohammad Hashem
collection PubMed
description The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study describes the isolation and characterization of a novel Salmonella Typhimurium phage ‘Arash’ from hospital wastewater in Leuven, Belgium. Arash has a myovirus morphology with a 95 nm capsid and a 140 nm tail. The host range of Arash is restricted to its isolation host. Approximately 86% of the phage particles are adsorbed to a host cell within 10 min. Arash has latent period of 65 min and burst size of 425 PFU/cell. Arash has a dsDNA genome of 180,819 bp with GC content of 53.02% with no similarities to any characterized phages, suggesting Arash as a novel species in the novel ‘Arashvirus’ genus. Arash carries no apparent lysogeny-, antibiotic resistance- nor virulence-related genes. Proteome analysis revealed 116 proteins as part of the mature phage particles of which 27 could be assigned a function. Therefore, the present findings shed light on the morphological, microbiological and genomic characteristics of Arash and suggest its potential application as therapeutic and/or biocontrol agent. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03056-9.
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spelling pubmed-105858452023-10-20 Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash Yousefi, Mohammad Hashem Wagemans, Jeroen Shekarforoush, Seyed Shahram Vallino, Marta Pozhydaieva, Nadiia Höfer, Katharina Lavigne, Rob Hosseinzadeh, Saeid BMC Microbiol Research The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study describes the isolation and characterization of a novel Salmonella Typhimurium phage ‘Arash’ from hospital wastewater in Leuven, Belgium. Arash has a myovirus morphology with a 95 nm capsid and a 140 nm tail. The host range of Arash is restricted to its isolation host. Approximately 86% of the phage particles are adsorbed to a host cell within 10 min. Arash has latent period of 65 min and burst size of 425 PFU/cell. Arash has a dsDNA genome of 180,819 bp with GC content of 53.02% with no similarities to any characterized phages, suggesting Arash as a novel species in the novel ‘Arashvirus’ genus. Arash carries no apparent lysogeny-, antibiotic resistance- nor virulence-related genes. Proteome analysis revealed 116 proteins as part of the mature phage particles of which 27 could be assigned a function. Therefore, the present findings shed light on the morphological, microbiological and genomic characteristics of Arash and suggest its potential application as therapeutic and/or biocontrol agent. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03056-9. BioMed Central 2023-10-19 /pmc/articles/PMC10585845/ /pubmed/37858092 http://dx.doi.org/10.1186/s12866-023-03056-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yousefi, Mohammad Hashem
Wagemans, Jeroen
Shekarforoush, Seyed Shahram
Vallino, Marta
Pozhydaieva, Nadiia
Höfer, Katharina
Lavigne, Rob
Hosseinzadeh, Saeid
Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
title Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
title_full Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
title_fullStr Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
title_full_unstemmed Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
title_short Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash
title_sort isolation and molecular characterization of the salmonella typhimurium orphan phage arash
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585845/
https://www.ncbi.nlm.nih.gov/pubmed/37858092
http://dx.doi.org/10.1186/s12866-023-03056-9
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