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Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection

BACKGROUND: Shrimp cultured in a biofloc system (BFS) have a lower disease incidence than those farmed in a water exchange system (WES). Although a number of studies have reported that the gut bacterial community induced by BFS is highly associated with shrimp disease resistance, the causal relation...

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Autores principales: Guo, Haipeng, Fu, Xuezhi, He, Jikun, Wang, Ruoyu, Yan, Mengchen, Wang, Jing, Dong, Pengsheng, Huang, Lei, Zhang, Demin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585862/
https://www.ncbi.nlm.nih.gov/pubmed/37858205
http://dx.doi.org/10.1186/s40168-023-01663-2
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author Guo, Haipeng
Fu, Xuezhi
He, Jikun
Wang, Ruoyu
Yan, Mengchen
Wang, Jing
Dong, Pengsheng
Huang, Lei
Zhang, Demin
author_facet Guo, Haipeng
Fu, Xuezhi
He, Jikun
Wang, Ruoyu
Yan, Mengchen
Wang, Jing
Dong, Pengsheng
Huang, Lei
Zhang, Demin
author_sort Guo, Haipeng
collection PubMed
description BACKGROUND: Shrimp cultured in a biofloc system (BFS) have a lower disease incidence than those farmed in a water exchange system (WES). Although a number of studies have reported that the gut bacterial community induced by BFS is highly associated with shrimp disease resistance, the causal relationship remains unknown. Here, the promotive roles of gut bacterial community induced by BFS in pathogenic Vibrio infection resistance and its potential micro-ecological and physiological mechanisms were investigated by gut bacterial consortium transplantation and synthetic community (SynCom) construction. RESULTS: The BFS induced a more stable and resistant gut bacterial community, and significantly enriched some beneficial bacterial taxa, such as Paracoccus, Ruegeria, Microbacterium, Demequina, and Tenacibaculum. Transplantation of a gut bacterial consortium from BFS shrimp (Enrich(BFS)) greatly enhanced the stability of the bacterial community and resistance against pathogenic V. parahaemolyticus infection in WES shrimp, while transplantation of a gut bacterial consortium from WES shrimp significantly disrupted the bacterial community and increased pathogen susceptibility in both WES and BFS shrimp. The addition of Enrich(BFS) in shrimp postlarvae also improved the pathogen resistance through increasing the relative abundances of beneficial bacterial taxa and stability of bacterial community. The corresponding strains of five beneficial bacterial taxa enriched in BFS shrimp were isolated to construct a SynCom(BFS). The addition of SynCom(BFS) could not only suppress disease development, but also improve shrimp growth, boost the digestive and immune activities, and restore health in diseased shrimp. Furthermore, the strains of SynCom(BFS) well colonized shrimp gut to maintain a high stability of bacterial community. CONCLUSIONS: Our study reveals an important role for native microbiota in protecting shrimp from bacterial pathogens and provides a micro-ecological regulation strategy towards the development of probiotics to ameliorate aquatic animal diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-023-01663-2.
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spelling pubmed-105858622023-10-20 Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection Guo, Haipeng Fu, Xuezhi He, Jikun Wang, Ruoyu Yan, Mengchen Wang, Jing Dong, Pengsheng Huang, Lei Zhang, Demin Microbiome Research BACKGROUND: Shrimp cultured in a biofloc system (BFS) have a lower disease incidence than those farmed in a water exchange system (WES). Although a number of studies have reported that the gut bacterial community induced by BFS is highly associated with shrimp disease resistance, the causal relationship remains unknown. Here, the promotive roles of gut bacterial community induced by BFS in pathogenic Vibrio infection resistance and its potential micro-ecological and physiological mechanisms were investigated by gut bacterial consortium transplantation and synthetic community (SynCom) construction. RESULTS: The BFS induced a more stable and resistant gut bacterial community, and significantly enriched some beneficial bacterial taxa, such as Paracoccus, Ruegeria, Microbacterium, Demequina, and Tenacibaculum. Transplantation of a gut bacterial consortium from BFS shrimp (Enrich(BFS)) greatly enhanced the stability of the bacterial community and resistance against pathogenic V. parahaemolyticus infection in WES shrimp, while transplantation of a gut bacterial consortium from WES shrimp significantly disrupted the bacterial community and increased pathogen susceptibility in both WES and BFS shrimp. The addition of Enrich(BFS) in shrimp postlarvae also improved the pathogen resistance through increasing the relative abundances of beneficial bacterial taxa and stability of bacterial community. The corresponding strains of five beneficial bacterial taxa enriched in BFS shrimp were isolated to construct a SynCom(BFS). The addition of SynCom(BFS) could not only suppress disease development, but also improve shrimp growth, boost the digestive and immune activities, and restore health in diseased shrimp. Furthermore, the strains of SynCom(BFS) well colonized shrimp gut to maintain a high stability of bacterial community. CONCLUSIONS: Our study reveals an important role for native microbiota in protecting shrimp from bacterial pathogens and provides a micro-ecological regulation strategy towards the development of probiotics to ameliorate aquatic animal diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-023-01663-2. BioMed Central 2023-10-19 /pmc/articles/PMC10585862/ /pubmed/37858205 http://dx.doi.org/10.1186/s40168-023-01663-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Guo, Haipeng
Fu, Xuezhi
He, Jikun
Wang, Ruoyu
Yan, Mengchen
Wang, Jing
Dong, Pengsheng
Huang, Lei
Zhang, Demin
Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection
title Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection
title_full Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection
title_fullStr Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection
title_full_unstemmed Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection
title_short Gut bacterial consortium enriched in a biofloc system protects shrimp against Vibrio parahaemolyticus infection
title_sort gut bacterial consortium enriched in a biofloc system protects shrimp against vibrio parahaemolyticus infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585862/
https://www.ncbi.nlm.nih.gov/pubmed/37858205
http://dx.doi.org/10.1186/s40168-023-01663-2
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