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Identification of mitochondria-related action targets of quercetin in melanoma cells
Melanoma is a complex and genetically heterogeneous malignant tumor with high rates of mortality. Although current therapies provide a short-term clinical benefit, they are unable to cure the majority of patients with metastatic melanoma. Therefore, the investigation of pathological mechanisms and t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586065/ https://www.ncbi.nlm.nih.gov/pubmed/37869567 http://dx.doi.org/10.1080/23802359.2023.2268775 |
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author | Xi, Qing Li, Li Yang, Yongjie Li, Liubing Zhang, Rongxin |
author_facet | Xi, Qing Li, Li Yang, Yongjie Li, Liubing Zhang, Rongxin |
author_sort | Xi, Qing |
collection | PubMed |
description | Melanoma is a complex and genetically heterogeneous malignant tumor with high rates of mortality. Although current therapies provide a short-term clinical benefit, they are unable to cure the majority of patients with metastatic melanoma. Therefore, the investigation of pathological mechanisms and the development of new therapy strategies for melanoma are of great significance. Quercetin can effectively inhibit tumor growth in various tumors. However, the exact action mechanisms of quercetin against melanoma have not been comprehensively clarified, which limits its application. Accumulating evidence has suggested that the dysfunction of mitochondria is closely linked to carcinogenesis, and a better understanding of the regulation of mitochondria-related genes will shed light on providing new therapies for melanoma. In this study, we performed RNA-seq from melanoma B16-F1 cells treated with quercetin versus controls and screened for differentially expressed genes (DEGs). GO and KEGG enrichment analyses were performed, and a protein–protein interaction (PPI) network was constructed. Combining the results of RNA-seq, molecular docking, and bioinformatics analysis, we found six mitochondria-related genes, BTG2, CP, LRIG1, CYP1A1, GBP2, and MBNL1, which might be targets of quercetin in melanoma and provide an available targeting therapy strategy for melanoma. |
format | Online Article Text |
id | pubmed-10586065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-105860652023-10-20 Identification of mitochondria-related action targets of quercetin in melanoma cells Xi, Qing Li, Li Yang, Yongjie Li, Liubing Zhang, Rongxin Mitochondrial DNA B Resour Rapid Communication Melanoma is a complex and genetically heterogeneous malignant tumor with high rates of mortality. Although current therapies provide a short-term clinical benefit, they are unable to cure the majority of patients with metastatic melanoma. Therefore, the investigation of pathological mechanisms and the development of new therapy strategies for melanoma are of great significance. Quercetin can effectively inhibit tumor growth in various tumors. However, the exact action mechanisms of quercetin against melanoma have not been comprehensively clarified, which limits its application. Accumulating evidence has suggested that the dysfunction of mitochondria is closely linked to carcinogenesis, and a better understanding of the regulation of mitochondria-related genes will shed light on providing new therapies for melanoma. In this study, we performed RNA-seq from melanoma B16-F1 cells treated with quercetin versus controls and screened for differentially expressed genes (DEGs). GO and KEGG enrichment analyses were performed, and a protein–protein interaction (PPI) network was constructed. Combining the results of RNA-seq, molecular docking, and bioinformatics analysis, we found six mitochondria-related genes, BTG2, CP, LRIG1, CYP1A1, GBP2, and MBNL1, which might be targets of quercetin in melanoma and provide an available targeting therapy strategy for melanoma. Taylor & Francis 2023-10-18 /pmc/articles/PMC10586065/ /pubmed/37869567 http://dx.doi.org/10.1080/23802359.2023.2268775 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Rapid Communication Xi, Qing Li, Li Yang, Yongjie Li, Liubing Zhang, Rongxin Identification of mitochondria-related action targets of quercetin in melanoma cells |
title | Identification of mitochondria-related action targets of quercetin in melanoma cells |
title_full | Identification of mitochondria-related action targets of quercetin in melanoma cells |
title_fullStr | Identification of mitochondria-related action targets of quercetin in melanoma cells |
title_full_unstemmed | Identification of mitochondria-related action targets of quercetin in melanoma cells |
title_short | Identification of mitochondria-related action targets of quercetin in melanoma cells |
title_sort | identification of mitochondria-related action targets of quercetin in melanoma cells |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586065/ https://www.ncbi.nlm.nih.gov/pubmed/37869567 http://dx.doi.org/10.1080/23802359.2023.2268775 |
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