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Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation

Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this i...

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Detalles Bibliográficos
Autores principales: Legan, Andrew Wesley, Mehl, Hillary L., Varaksa, Alex, Callicott, Kenneth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586124/
https://www.ncbi.nlm.nih.gov/pubmed/37728564
http://dx.doi.org/10.1128/MRA.00527-23
Descripción
Sumario:Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.