Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation

Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this i...

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Detalles Bibliográficos
Autores principales: Legan, Andrew Wesley, Mehl, Hillary L., Varaksa, Alex, Callicott, Kenneth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Omics Data Sets
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586124/
https://www.ncbi.nlm.nih.gov/pubmed/37728564
http://dx.doi.org/10.1128/MRA.00527-23
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author Legan, Andrew Wesley
Mehl, Hillary L.
Varaksa, Alex
Callicott, Kenneth A.
author_facet Legan, Andrew Wesley
Mehl, Hillary L.
Varaksa, Alex
Callicott, Kenneth A.
author_sort Legan, Andrew Wesley
collection PubMed
description Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.
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spelling pubmed-105861242023-10-20 Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation Legan, Andrew Wesley Mehl, Hillary L. Varaksa, Alex Callicott, Kenneth A. Microbiol Resour Announc Omics Data Sets Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing. American Society for Microbiology 2023-09-20 /pmc/articles/PMC10586124/ /pubmed/37728564 http://dx.doi.org/10.1128/MRA.00527-23 Text en https://doi.org/10.1128/AuthorWarrantyLicense.v1This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
spellingShingle Omics Data Sets
Legan, Andrew Wesley
Mehl, Hillary L.
Varaksa, Alex
Callicott, Kenneth A.
Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation
title Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation
title_full Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation
title_fullStr Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation
title_full_unstemmed Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation
title_short Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation
title_sort nanopore pcr-cdna sequencing of the biocontrol isolate aspergillus flavus af36 (nrrl 18543) informs gene annotation
topic Omics Data Sets
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586124/
https://www.ncbi.nlm.nih.gov/pubmed/37728564
http://dx.doi.org/10.1128/MRA.00527-23
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