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Maximizing the performance of protein-based fluorescent biosensors
Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the imaging of biological processes in the context of cells, tissues, or live animals. Though widely used in biological research, practically all existing biosensors are far from ideal in terms of their performance,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586770/ https://www.ncbi.nlm.nih.gov/pubmed/37431791 http://dx.doi.org/10.1042/BST20221413 |
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author | Chai, Fu Cheng, Dazhou Nasu, Yusuke Terai, Takuya Campbell, Robert E. |
author_facet | Chai, Fu Cheng, Dazhou Nasu, Yusuke Terai, Takuya Campbell, Robert E. |
author_sort | Chai, Fu |
collection | PubMed |
description | Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the imaging of biological processes in the context of cells, tissues, or live animals. Though widely used in biological research, practically all existing biosensors are far from ideal in terms of their performance, properties, and applicability for multiplexed imaging. These limitations have inspired researchers to explore an increasing number of innovative and creative ways to improve and maximize biosensor performance. Such strategies include new molecular biology methods to develop promising biosensor prototypes, high throughput microfluidics-based directed evolution screening strategies, and improved ways to perform multiplexed imaging. Yet another approach is to effectively replace components of biosensors with self-labeling proteins, such as HaloTag, that enable the biocompatible incorporation of synthetic fluorophores or other ligands in cells or tissues. This mini-review will summarize and highlight recent innovations and strategies for enhancing the performance of FP-based biosensors for multiplexed imaging to advance the frontiers of research. |
format | Online Article Text |
id | pubmed-10586770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105867702023-10-20 Maximizing the performance of protein-based fluorescent biosensors Chai, Fu Cheng, Dazhou Nasu, Yusuke Terai, Takuya Campbell, Robert E. Biochem Soc Trans Review Articles Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the imaging of biological processes in the context of cells, tissues, or live animals. Though widely used in biological research, practically all existing biosensors are far from ideal in terms of their performance, properties, and applicability for multiplexed imaging. These limitations have inspired researchers to explore an increasing number of innovative and creative ways to improve and maximize biosensor performance. Such strategies include new molecular biology methods to develop promising biosensor prototypes, high throughput microfluidics-based directed evolution screening strategies, and improved ways to perform multiplexed imaging. Yet another approach is to effectively replace components of biosensors with self-labeling proteins, such as HaloTag, that enable the biocompatible incorporation of synthetic fluorophores or other ligands in cells or tissues. This mini-review will summarize and highlight recent innovations and strategies for enhancing the performance of FP-based biosensors for multiplexed imaging to advance the frontiers of research. Portland Press Ltd. 2023-08-31 2023-07-11 /pmc/articles/PMC10586770/ /pubmed/37431791 http://dx.doi.org/10.1042/BST20221413 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of University of Tokyo in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with Individual. |
spellingShingle | Review Articles Chai, Fu Cheng, Dazhou Nasu, Yusuke Terai, Takuya Campbell, Robert E. Maximizing the performance of protein-based fluorescent biosensors |
title | Maximizing the performance of protein-based fluorescent biosensors |
title_full | Maximizing the performance of protein-based fluorescent biosensors |
title_fullStr | Maximizing the performance of protein-based fluorescent biosensors |
title_full_unstemmed | Maximizing the performance of protein-based fluorescent biosensors |
title_short | Maximizing the performance of protein-based fluorescent biosensors |
title_sort | maximizing the performance of protein-based fluorescent biosensors |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586770/ https://www.ncbi.nlm.nih.gov/pubmed/37431791 http://dx.doi.org/10.1042/BST20221413 |
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