Cargando…

Maximizing the performance of protein-based fluorescent biosensors

Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the imaging of biological processes in the context of cells, tissues, or live animals. Though widely used in biological research, practically all existing biosensors are far from ideal in terms of their performance,...

Descripción completa

Detalles Bibliográficos
Autores principales: Chai, Fu, Cheng, Dazhou, Nasu, Yusuke, Terai, Takuya, Campbell, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586770/
https://www.ncbi.nlm.nih.gov/pubmed/37431791
http://dx.doi.org/10.1042/BST20221413
_version_ 1785123214223998976
author Chai, Fu
Cheng, Dazhou
Nasu, Yusuke
Terai, Takuya
Campbell, Robert E.
author_facet Chai, Fu
Cheng, Dazhou
Nasu, Yusuke
Terai, Takuya
Campbell, Robert E.
author_sort Chai, Fu
collection PubMed
description Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the imaging of biological processes in the context of cells, tissues, or live animals. Though widely used in biological research, practically all existing biosensors are far from ideal in terms of their performance, properties, and applicability for multiplexed imaging. These limitations have inspired researchers to explore an increasing number of innovative and creative ways to improve and maximize biosensor performance. Such strategies include new molecular biology methods to develop promising biosensor prototypes, high throughput microfluidics-based directed evolution screening strategies, and improved ways to perform multiplexed imaging. Yet another approach is to effectively replace components of biosensors with self-labeling proteins, such as HaloTag, that enable the biocompatible incorporation of synthetic fluorophores or other ligands in cells or tissues. This mini-review will summarize and highlight recent innovations and strategies for enhancing the performance of FP-based biosensors for multiplexed imaging to advance the frontiers of research.
format Online
Article
Text
id pubmed-10586770
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-105867702023-10-20 Maximizing the performance of protein-based fluorescent biosensors Chai, Fu Cheng, Dazhou Nasu, Yusuke Terai, Takuya Campbell, Robert E. Biochem Soc Trans Review Articles Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the imaging of biological processes in the context of cells, tissues, or live animals. Though widely used in biological research, practically all existing biosensors are far from ideal in terms of their performance, properties, and applicability for multiplexed imaging. These limitations have inspired researchers to explore an increasing number of innovative and creative ways to improve and maximize biosensor performance. Such strategies include new molecular biology methods to develop promising biosensor prototypes, high throughput microfluidics-based directed evolution screening strategies, and improved ways to perform multiplexed imaging. Yet another approach is to effectively replace components of biosensors with self-labeling proteins, such as HaloTag, that enable the biocompatible incorporation of synthetic fluorophores or other ligands in cells or tissues. This mini-review will summarize and highlight recent innovations and strategies for enhancing the performance of FP-based biosensors for multiplexed imaging to advance the frontiers of research. Portland Press Ltd. 2023-08-31 2023-07-11 /pmc/articles/PMC10586770/ /pubmed/37431791 http://dx.doi.org/10.1042/BST20221413 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of University of Tokyo in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with Individual.
spellingShingle Review Articles
Chai, Fu
Cheng, Dazhou
Nasu, Yusuke
Terai, Takuya
Campbell, Robert E.
Maximizing the performance of protein-based fluorescent biosensors
title Maximizing the performance of protein-based fluorescent biosensors
title_full Maximizing the performance of protein-based fluorescent biosensors
title_fullStr Maximizing the performance of protein-based fluorescent biosensors
title_full_unstemmed Maximizing the performance of protein-based fluorescent biosensors
title_short Maximizing the performance of protein-based fluorescent biosensors
title_sort maximizing the performance of protein-based fluorescent biosensors
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586770/
https://www.ncbi.nlm.nih.gov/pubmed/37431791
http://dx.doi.org/10.1042/BST20221413
work_keys_str_mv AT chaifu maximizingtheperformanceofproteinbasedfluorescentbiosensors
AT chengdazhou maximizingtheperformanceofproteinbasedfluorescentbiosensors
AT nasuyusuke maximizingtheperformanceofproteinbasedfluorescentbiosensors
AT teraitakuya maximizingtheperformanceofproteinbasedfluorescentbiosensors
AT campbellroberte maximizingtheperformanceofproteinbasedfluorescentbiosensors