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Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae

How animals respond to repeatedly applied stimuli, and how animals respond to mechanical stimuli in particular, are important questions in behavioral neuroscience. We study adaptation to repeated mechanical agitation using the Drosophila larva. Vertical vibration stimuli elicit a discrete set of res...

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Autores principales: Berne, Alexander, Zhang, Tom, Shomar, Joseph, Ferrer, Anggie J, Valdes, Aaron, Ohyama, Tomoko, Klein, Mason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586805/
https://www.ncbi.nlm.nih.gov/pubmed/37855833
http://dx.doi.org/10.7554/eLife.69205
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author Berne, Alexander
Zhang, Tom
Shomar, Joseph
Ferrer, Anggie J
Valdes, Aaron
Ohyama, Tomoko
Klein, Mason
author_facet Berne, Alexander
Zhang, Tom
Shomar, Joseph
Ferrer, Anggie J
Valdes, Aaron
Ohyama, Tomoko
Klein, Mason
author_sort Berne, Alexander
collection PubMed
description How animals respond to repeatedly applied stimuli, and how animals respond to mechanical stimuli in particular, are important questions in behavioral neuroscience. We study adaptation to repeated mechanical agitation using the Drosophila larva. Vertical vibration stimuli elicit a discrete set of responses in crawling larvae: continuation, pause, turn, and reversal. Through high-throughput larva tracking, we characterize how the likelihood of each response depends on vibration intensity and on the timing of repeated vibration pulses. By examining transitions between behavioral states at the population and individual levels, we investigate how the animals habituate to the stimulus patterns. We identify time constants associated with desensitization to prolonged vibration, with re-sensitization during removal of a stimulus, and additional layers of habituation that operate in the overall response. Known memory-deficient mutants exhibit distinct behavior profiles and habituation time constants. An analogous simple electrical circuit suggests possible neural and molecular processes behind adaptive behavior.
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spelling pubmed-105868052023-10-20 Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae Berne, Alexander Zhang, Tom Shomar, Joseph Ferrer, Anggie J Valdes, Aaron Ohyama, Tomoko Klein, Mason eLife Neuroscience How animals respond to repeatedly applied stimuli, and how animals respond to mechanical stimuli in particular, are important questions in behavioral neuroscience. We study adaptation to repeated mechanical agitation using the Drosophila larva. Vertical vibration stimuli elicit a discrete set of responses in crawling larvae: continuation, pause, turn, and reversal. Through high-throughput larva tracking, we characterize how the likelihood of each response depends on vibration intensity and on the timing of repeated vibration pulses. By examining transitions between behavioral states at the population and individual levels, we investigate how the animals habituate to the stimulus patterns. We identify time constants associated with desensitization to prolonged vibration, with re-sensitization during removal of a stimulus, and additional layers of habituation that operate in the overall response. Known memory-deficient mutants exhibit distinct behavior profiles and habituation time constants. An analogous simple electrical circuit suggests possible neural and molecular processes behind adaptive behavior. eLife Sciences Publications, Ltd 2023-10-19 /pmc/articles/PMC10586805/ /pubmed/37855833 http://dx.doi.org/10.7554/eLife.69205 Text en © 2023, Berne et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Berne, Alexander
Zhang, Tom
Shomar, Joseph
Ferrer, Anggie J
Valdes, Aaron
Ohyama, Tomoko
Klein, Mason
Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae
title Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae
title_full Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae
title_fullStr Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae
title_full_unstemmed Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae
title_short Mechanical vibration patterns elicit behavioral transitions and habituation in crawling Drosophila larvae
title_sort mechanical vibration patterns elicit behavioral transitions and habituation in crawling drosophila larvae
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586805/
https://www.ncbi.nlm.nih.gov/pubmed/37855833
http://dx.doi.org/10.7554/eLife.69205
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