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Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD

Metabolic dysfunction–associated steatotic liver disease (MASLD) is a chronic liver disease prevalent worldwide, with an increasing incidence associated with obesity, diabetes, and metabolic syndrome. The progression of MASLD to metabolic dysfunction–associated steatohepatitis (MASH) poses a pressin...

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Autores principales: Bai, Jing, Zhu, Lun, Mi, Wanqi, Gao, Zhengzheng, Ouyang, Minyue, Sheng, Wanlu, Song, Lin, Bao, Lidao, Ma, Yuheng, Xu, Yingqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586828/
https://www.ncbi.nlm.nih.gov/pubmed/37851406
http://dx.doi.org/10.1097/HC9.0000000000000298
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author Bai, Jing
Zhu, Lun
Mi, Wanqi
Gao, Zhengzheng
Ouyang, Minyue
Sheng, Wanlu
Song, Lin
Bao, Lidao
Ma, Yuheng
Xu, Yingqi
author_facet Bai, Jing
Zhu, Lun
Mi, Wanqi
Gao, Zhengzheng
Ouyang, Minyue
Sheng, Wanlu
Song, Lin
Bao, Lidao
Ma, Yuheng
Xu, Yingqi
author_sort Bai, Jing
collection PubMed
description Metabolic dysfunction–associated steatotic liver disease (MASLD) is a chronic liver disease prevalent worldwide, with an increasing incidence associated with obesity, diabetes, and metabolic syndrome. The progression of MASLD to metabolic dysfunction–associated steatohepatitis (MASH) poses a pressing health concern, highlighting the significance of accurately identifying MASLD and its progression to MASH as a primary challenge in the field. In this study, a systematic integration of 66 immune cell types was conducted. Comprehensive analyses were performed on bulk, single-cell RNA-Seq, and clinical data to investigate the immune cell types implicated in MASLD progression thoroughly. Multiple approaches, including immune infiltration, gene expression trend analysis, weighted gene coexpression network analysis, and 4 machine learning algorithms, were used to examine the dynamic changes in genes and immune cells during MASLD progression. C-X-C motif chemokine receptor 4 and dedicator of cytokinesis 8 have been identified as potential diagnostic biomarkers for MASLD progression. Furthermore, cell communication analysis at the single-cell level revealed that the involvement of C-X-C motif chemokine receptor 4 and dedicator of cytokinesis 8 in MASLD progression is mediated through their influence on T cells. Overall, our study identified vital immune cells and a 2-gene diagnostic signature for the progression of MASLD, providing a new perspective on the diagnosis and immune-related molecular mechanisms of MASLD. These findings have important implications for developing innovative diagnostic tools and therapies for MASLD.
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spelling pubmed-105868282023-10-20 Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD Bai, Jing Zhu, Lun Mi, Wanqi Gao, Zhengzheng Ouyang, Minyue Sheng, Wanlu Song, Lin Bao, Lidao Ma, Yuheng Xu, Yingqi Hepatol Commun Original Article Metabolic dysfunction–associated steatotic liver disease (MASLD) is a chronic liver disease prevalent worldwide, with an increasing incidence associated with obesity, diabetes, and metabolic syndrome. The progression of MASLD to metabolic dysfunction–associated steatohepatitis (MASH) poses a pressing health concern, highlighting the significance of accurately identifying MASLD and its progression to MASH as a primary challenge in the field. In this study, a systematic integration of 66 immune cell types was conducted. Comprehensive analyses were performed on bulk, single-cell RNA-Seq, and clinical data to investigate the immune cell types implicated in MASLD progression thoroughly. Multiple approaches, including immune infiltration, gene expression trend analysis, weighted gene coexpression network analysis, and 4 machine learning algorithms, were used to examine the dynamic changes in genes and immune cells during MASLD progression. C-X-C motif chemokine receptor 4 and dedicator of cytokinesis 8 have been identified as potential diagnostic biomarkers for MASLD progression. Furthermore, cell communication analysis at the single-cell level revealed that the involvement of C-X-C motif chemokine receptor 4 and dedicator of cytokinesis 8 in MASLD progression is mediated through their influence on T cells. Overall, our study identified vital immune cells and a 2-gene diagnostic signature for the progression of MASLD, providing a new perspective on the diagnosis and immune-related molecular mechanisms of MASLD. These findings have important implications for developing innovative diagnostic tools and therapies for MASLD. Lippincott Williams & Wilkins 2023-10-18 /pmc/articles/PMC10586828/ /pubmed/37851406 http://dx.doi.org/10.1097/HC9.0000000000000298 Text en Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Association for the Study of Liver Diseases. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
Bai, Jing
Zhu, Lun
Mi, Wanqi
Gao, Zhengzheng
Ouyang, Minyue
Sheng, Wanlu
Song, Lin
Bao, Lidao
Ma, Yuheng
Xu, Yingqi
Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD
title Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD
title_full Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD
title_fullStr Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD
title_full_unstemmed Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD
title_short Multiscale integrative analyses unveil immune-related diagnostic signature for the progression of MASLD
title_sort multiscale integrative analyses unveil immune-related diagnostic signature for the progression of masld
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586828/
https://www.ncbi.nlm.nih.gov/pubmed/37851406
http://dx.doi.org/10.1097/HC9.0000000000000298
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