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Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation
We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—the initial...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587353/ https://www.ncbi.nlm.nih.gov/pubmed/37857684 http://dx.doi.org/10.1038/s41598-023-45018-1 |
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author | Bagramyan, Arutyun Lin, Charles P. |
author_facet | Bagramyan, Arutyun Lin, Charles P. |
author_sort | Bagramyan, Arutyun |
collection | PubMed |
description | We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—the initial steps in leukocyte recruitment that is a hallmark of inflammation. Using the mOBM system, we studied the leukocyte-endothelial interactions in healthy and locally inflamed tissue and observed drastic changes in leukocyte movement (velocity and displacement profile). Our findings suggest that real-time imaging of leukocyte dynamics can provide new diagnostic insights (assessment of inflammation, temporal progression of disease, evaluation of therapeutic response, etc.) that are not available using conventional static parameters such as cell number and morphology. |
format | Online Article Text |
id | pubmed-10587353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105873532023-10-21 Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation Bagramyan, Arutyun Lin, Charles P. Sci Rep Article We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—the initial steps in leukocyte recruitment that is a hallmark of inflammation. Using the mOBM system, we studied the leukocyte-endothelial interactions in healthy and locally inflamed tissue and observed drastic changes in leukocyte movement (velocity and displacement profile). Our findings suggest that real-time imaging of leukocyte dynamics can provide new diagnostic insights (assessment of inflammation, temporal progression of disease, evaluation of therapeutic response, etc.) that are not available using conventional static parameters such as cell number and morphology. Nature Publishing Group UK 2023-10-19 /pmc/articles/PMC10587353/ /pubmed/37857684 http://dx.doi.org/10.1038/s41598-023-45018-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bagramyan, Arutyun Lin, Charles P. Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
title | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
title_full | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
title_fullStr | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
title_full_unstemmed | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
title_short | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
title_sort | miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587353/ https://www.ncbi.nlm.nih.gov/pubmed/37857684 http://dx.doi.org/10.1038/s41598-023-45018-1 |
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