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Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice

This study aims to explore the molecular regulatory mechanisms of acute exercise in the skeletal muscle of mice. Male C57BL/6 mice were randomly assigned to the control group, and the exercise group, which were sacrificed immediately after an acute bout of exercise. The study was conducted to invest...

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Autores principales: Ye, Xing, Liu, Renyi, Qiao, Zhixian, Chai, Xiaocui, Wang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587468/
https://www.ncbi.nlm.nih.gov/pubmed/37869715
http://dx.doi.org/10.3389/fphys.2023.1273342
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author Ye, Xing
Liu, Renyi
Qiao, Zhixian
Chai, Xiaocui
Wang, Yan
author_facet Ye, Xing
Liu, Renyi
Qiao, Zhixian
Chai, Xiaocui
Wang, Yan
author_sort Ye, Xing
collection PubMed
description This study aims to explore the molecular regulatory mechanisms of acute exercise in the skeletal muscle of mice. Male C57BL/6 mice were randomly assigned to the control group, and the exercise group, which were sacrificed immediately after an acute bout of exercise. The study was conducted to investigate the metabolic and transcriptional profiling in the quadriceps muscles of mice. The results demonstrated the identification of 34 differentially expressed metabolites (DEMs), with 28 upregulated and 6 downregulated, between the two groups. Metabolic pathway analysis revealed that these DEMs were primarily enriched in several, including the citrate cycle, propanoate metabolism, and lysine degradation pathways. In addition, the results showed a total of 245 differentially expressed genes (DEGs), with 155 genes upregulated and 90 genes downregulated. KEGG analysis indicated that these DEGs were mainly enriched in various pathways such as ubiquitin mediated proteolysis and FoxO signaling pathway. Furthermore, the analysis revealed significant enrichment of DEMs and DEGs in signaling pathways such as protein digestion and absorption, ferroptosis signaling pathway. In summary, the identified multiple metabolic pathways and signaling pathways were involved in the exercise-induced physiological regulation of skeletal muscle, such as the TCA cycle, oxidative phosphorylation, protein digestion and absorption, the FoxO signaling pathway, ubiquitin mediated proteolysis, ferroptosis signaling pathway, and the upregulation of KLF-15, FoxO1, MAFbx, and MuRF1 expression could play a critical role in enhancing skeletal muscle proteolysis.
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spelling pubmed-105874682023-10-21 Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice Ye, Xing Liu, Renyi Qiao, Zhixian Chai, Xiaocui Wang, Yan Front Physiol Physiology This study aims to explore the molecular regulatory mechanisms of acute exercise in the skeletal muscle of mice. Male C57BL/6 mice were randomly assigned to the control group, and the exercise group, which were sacrificed immediately after an acute bout of exercise. The study was conducted to investigate the metabolic and transcriptional profiling in the quadriceps muscles of mice. The results demonstrated the identification of 34 differentially expressed metabolites (DEMs), with 28 upregulated and 6 downregulated, between the two groups. Metabolic pathway analysis revealed that these DEMs were primarily enriched in several, including the citrate cycle, propanoate metabolism, and lysine degradation pathways. In addition, the results showed a total of 245 differentially expressed genes (DEGs), with 155 genes upregulated and 90 genes downregulated. KEGG analysis indicated that these DEGs were mainly enriched in various pathways such as ubiquitin mediated proteolysis and FoxO signaling pathway. Furthermore, the analysis revealed significant enrichment of DEMs and DEGs in signaling pathways such as protein digestion and absorption, ferroptosis signaling pathway. In summary, the identified multiple metabolic pathways and signaling pathways were involved in the exercise-induced physiological regulation of skeletal muscle, such as the TCA cycle, oxidative phosphorylation, protein digestion and absorption, the FoxO signaling pathway, ubiquitin mediated proteolysis, ferroptosis signaling pathway, and the upregulation of KLF-15, FoxO1, MAFbx, and MuRF1 expression could play a critical role in enhancing skeletal muscle proteolysis. Frontiers Media S.A. 2023-10-06 /pmc/articles/PMC10587468/ /pubmed/37869715 http://dx.doi.org/10.3389/fphys.2023.1273342 Text en Copyright © 2023 Ye, Liu, Qiao, Chai and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Ye, Xing
Liu, Renyi
Qiao, Zhixian
Chai, Xiaocui
Wang, Yan
Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
title Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
title_full Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
title_fullStr Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
title_full_unstemmed Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
title_short Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
title_sort integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587468/
https://www.ncbi.nlm.nih.gov/pubmed/37869715
http://dx.doi.org/10.3389/fphys.2023.1273342
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