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Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice
This study aims to explore the molecular regulatory mechanisms of acute exercise in the skeletal muscle of mice. Male C57BL/6 mice were randomly assigned to the control group, and the exercise group, which were sacrificed immediately after an acute bout of exercise. The study was conducted to invest...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587468/ https://www.ncbi.nlm.nih.gov/pubmed/37869715 http://dx.doi.org/10.3389/fphys.2023.1273342 |
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author | Ye, Xing Liu, Renyi Qiao, Zhixian Chai, Xiaocui Wang, Yan |
author_facet | Ye, Xing Liu, Renyi Qiao, Zhixian Chai, Xiaocui Wang, Yan |
author_sort | Ye, Xing |
collection | PubMed |
description | This study aims to explore the molecular regulatory mechanisms of acute exercise in the skeletal muscle of mice. Male C57BL/6 mice were randomly assigned to the control group, and the exercise group, which were sacrificed immediately after an acute bout of exercise. The study was conducted to investigate the metabolic and transcriptional profiling in the quadriceps muscles of mice. The results demonstrated the identification of 34 differentially expressed metabolites (DEMs), with 28 upregulated and 6 downregulated, between the two groups. Metabolic pathway analysis revealed that these DEMs were primarily enriched in several, including the citrate cycle, propanoate metabolism, and lysine degradation pathways. In addition, the results showed a total of 245 differentially expressed genes (DEGs), with 155 genes upregulated and 90 genes downregulated. KEGG analysis indicated that these DEGs were mainly enriched in various pathways such as ubiquitin mediated proteolysis and FoxO signaling pathway. Furthermore, the analysis revealed significant enrichment of DEMs and DEGs in signaling pathways such as protein digestion and absorption, ferroptosis signaling pathway. In summary, the identified multiple metabolic pathways and signaling pathways were involved in the exercise-induced physiological regulation of skeletal muscle, such as the TCA cycle, oxidative phosphorylation, protein digestion and absorption, the FoxO signaling pathway, ubiquitin mediated proteolysis, ferroptosis signaling pathway, and the upregulation of KLF-15, FoxO1, MAFbx, and MuRF1 expression could play a critical role in enhancing skeletal muscle proteolysis. |
format | Online Article Text |
id | pubmed-10587468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105874682023-10-21 Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice Ye, Xing Liu, Renyi Qiao, Zhixian Chai, Xiaocui Wang, Yan Front Physiol Physiology This study aims to explore the molecular regulatory mechanisms of acute exercise in the skeletal muscle of mice. Male C57BL/6 mice were randomly assigned to the control group, and the exercise group, which were sacrificed immediately after an acute bout of exercise. The study was conducted to investigate the metabolic and transcriptional profiling in the quadriceps muscles of mice. The results demonstrated the identification of 34 differentially expressed metabolites (DEMs), with 28 upregulated and 6 downregulated, between the two groups. Metabolic pathway analysis revealed that these DEMs were primarily enriched in several, including the citrate cycle, propanoate metabolism, and lysine degradation pathways. In addition, the results showed a total of 245 differentially expressed genes (DEGs), with 155 genes upregulated and 90 genes downregulated. KEGG analysis indicated that these DEGs were mainly enriched in various pathways such as ubiquitin mediated proteolysis and FoxO signaling pathway. Furthermore, the analysis revealed significant enrichment of DEMs and DEGs in signaling pathways such as protein digestion and absorption, ferroptosis signaling pathway. In summary, the identified multiple metabolic pathways and signaling pathways were involved in the exercise-induced physiological regulation of skeletal muscle, such as the TCA cycle, oxidative phosphorylation, protein digestion and absorption, the FoxO signaling pathway, ubiquitin mediated proteolysis, ferroptosis signaling pathway, and the upregulation of KLF-15, FoxO1, MAFbx, and MuRF1 expression could play a critical role in enhancing skeletal muscle proteolysis. Frontiers Media S.A. 2023-10-06 /pmc/articles/PMC10587468/ /pubmed/37869715 http://dx.doi.org/10.3389/fphys.2023.1273342 Text en Copyright © 2023 Ye, Liu, Qiao, Chai and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Ye, Xing Liu, Renyi Qiao, Zhixian Chai, Xiaocui Wang, Yan Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
title | Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
title_full | Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
title_fullStr | Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
title_full_unstemmed | Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
title_short | Integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
title_sort | integrative profiling of metabolome and transcriptome of skeletal muscle after acute exercise intervention in mice |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587468/ https://www.ncbi.nlm.nih.gov/pubmed/37869715 http://dx.doi.org/10.3389/fphys.2023.1273342 |
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