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Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies

Organ supply remains inadequate to meet the needs of many patients who could benefit from allotransplantation. Xenotransplantation, the use of animals as organ donors, provides an opportunity to alleviate this challenge. Pigs are widely accepted as the ideal organ donor, but humans and nonhuman prim...

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Autores principales: Wang, Zheng-Yu, Reyes, Luz, Estrada, Jose, Burlak, Christopher, Gennuso, Victor Novara, Tector, Mosely O., Ho, Sam, Tector, Matt, Tector, A. Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587499/
https://www.ncbi.nlm.nih.gov/pubmed/37712913
http://dx.doi.org/10.4049/immunohorizons.2300056
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author Wang, Zheng-Yu
Reyes, Luz
Estrada, Jose
Burlak, Christopher
Gennuso, Victor Novara
Tector, Mosely O.
Ho, Sam
Tector, Matt
Tector, A. Joseph
author_facet Wang, Zheng-Yu
Reyes, Luz
Estrada, Jose
Burlak, Christopher
Gennuso, Victor Novara
Tector, Mosely O.
Ho, Sam
Tector, Matt
Tector, A. Joseph
author_sort Wang, Zheng-Yu
collection PubMed
description Organ supply remains inadequate to meet the needs of many patients who could benefit from allotransplantation. Xenotransplantation, the use of animals as organ donors, provides an opportunity to alleviate this challenge. Pigs are widely accepted as the ideal organ donor, but humans and nonhuman primates have strong humoral immune responses to porcine tissue. Although carbohydrate xenoantigens have been studied intensively, the primate Ab response also targets class I and class II swine leukocyte Ags (SLAs). Human Abs that recognize HLAs can cross-react with SLA molecules because epitopes can be shared across species. However, ∼15% of people may also exhibit Abs toward class II SLAs despite lacking Abs that also recognize class II HLAs. Here, we extend these studies to better understand human Ab responses toward class I SLAs. When tested against a panel of 18 unique class I SLA proteins, 14 of 52 sera samples collected from patients in need of an organ transplant contained Abs that bound class I SLAs. Class I SLA–reactive sera may contain IgM only, IgG, only, or IgM and IgG capable of recognizing the pig proteins. The presence of class I HLA–reactive Abs was not essential to generating anti–class I SLA Ig. Last, anti–class I SLA reactivity varied by serum; some recognized a single SLA allele, whereas others recognized multiple class I SLA proteins.
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spelling pubmed-105874992023-10-23 Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies Wang, Zheng-Yu Reyes, Luz Estrada, Jose Burlak, Christopher Gennuso, Victor Novara Tector, Mosely O. Ho, Sam Tector, Matt Tector, A. Joseph Immunohorizons Adaptive Immunity Organ supply remains inadequate to meet the needs of many patients who could benefit from allotransplantation. Xenotransplantation, the use of animals as organ donors, provides an opportunity to alleviate this challenge. Pigs are widely accepted as the ideal organ donor, but humans and nonhuman primates have strong humoral immune responses to porcine tissue. Although carbohydrate xenoantigens have been studied intensively, the primate Ab response also targets class I and class II swine leukocyte Ags (SLAs). Human Abs that recognize HLAs can cross-react with SLA molecules because epitopes can be shared across species. However, ∼15% of people may also exhibit Abs toward class II SLAs despite lacking Abs that also recognize class II HLAs. Here, we extend these studies to better understand human Ab responses toward class I SLAs. When tested against a panel of 18 unique class I SLA proteins, 14 of 52 sera samples collected from patients in need of an organ transplant contained Abs that bound class I SLAs. Class I SLA–reactive sera may contain IgM only, IgG, only, or IgM and IgG capable of recognizing the pig proteins. The presence of class I HLA–reactive Abs was not essential to generating anti–class I SLA Ig. Last, anti–class I SLA reactivity varied by serum; some recognized a single SLA allele, whereas others recognized multiple class I SLA proteins. AAI 2023-09-15 /pmc/articles/PMC10587499/ /pubmed/37712913 http://dx.doi.org/10.4049/immunohorizons.2300056 Text en Copyright © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the CC BY 4.0 Unported license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Adaptive Immunity
Wang, Zheng-Yu
Reyes, Luz
Estrada, Jose
Burlak, Christopher
Gennuso, Victor Novara
Tector, Mosely O.
Ho, Sam
Tector, Matt
Tector, A. Joseph
Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies
title Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies
title_full Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies
title_fullStr Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies
title_full_unstemmed Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies
title_short Patients on the Transplant Waiting List Have Anti-Swine Leukocyte Antigen Class I Antibodies
title_sort patients on the transplant waiting list have anti-swine leukocyte antigen class i antibodies
topic Adaptive Immunity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587499/
https://www.ncbi.nlm.nih.gov/pubmed/37712913
http://dx.doi.org/10.4049/immunohorizons.2300056
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