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Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population

Northern corn leaf blight (NCLB) is an economically important disease of maize. While the genetic architecture of NCLB has been well characterized, the pathogen is known to overcome currently deployed resistance genes, and the role of hormones in resistance to NCLB is an area of active research. The...

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Autores principales: Lipps, Sarah, Lipka, Alexander E., Mideros, Santiago, Jamann, Tiffany
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587583/
https://www.ncbi.nlm.nih.gov/pubmed/37868313
http://dx.doi.org/10.3389/fpls.2023.1272951
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author Lipps, Sarah
Lipka, Alexander E.
Mideros, Santiago
Jamann, Tiffany
author_facet Lipps, Sarah
Lipka, Alexander E.
Mideros, Santiago
Jamann, Tiffany
author_sort Lipps, Sarah
collection PubMed
description Northern corn leaf blight (NCLB) is an economically important disease of maize. While the genetic architecture of NCLB has been well characterized, the pathogen is known to overcome currently deployed resistance genes, and the role of hormones in resistance to NCLB is an area of active research. The objectives of the study were (i) to identify significant markers associated with resistance to NCLB, (ii) to identify metabolic pathways associated with NCLB resistance, and (iii) to examine role of ethylene in resistance to NCLB. We screened 252 lines from the exotic-derived double haploid BGEM maize population for resistance to NCLB in both field and greenhouse environments. We used a genome wide association study (GWAS) and stepwise regression to identify four markers associated with resistance, followed by a pathway association study tool (PAST) to identify important metabolic pathways associated with disease severity and incubation period. The ethylene synthesis pathway was significant for disease severity and incubation period. We conducted a greenhouse assay in which we inhibited ethylene to examine the role of ethylene in resistance to NCLB. We observed a significant increase in incubation period and a significant decrease in disease severity between plants treated with the ethylene inhibitor and mock-treated plants. Our study confirms the potential of the BGEM population as a source of novel alleles for resistance. We also confirm the role of ethylene in resistance to NCLB and contribute to the growing body of literature on ethylene and disease resistance in monocots.
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spelling pubmed-105875832023-10-21 Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population Lipps, Sarah Lipka, Alexander E. Mideros, Santiago Jamann, Tiffany Front Plant Sci Plant Science Northern corn leaf blight (NCLB) is an economically important disease of maize. While the genetic architecture of NCLB has been well characterized, the pathogen is known to overcome currently deployed resistance genes, and the role of hormones in resistance to NCLB is an area of active research. The objectives of the study were (i) to identify significant markers associated with resistance to NCLB, (ii) to identify metabolic pathways associated with NCLB resistance, and (iii) to examine role of ethylene in resistance to NCLB. We screened 252 lines from the exotic-derived double haploid BGEM maize population for resistance to NCLB in both field and greenhouse environments. We used a genome wide association study (GWAS) and stepwise regression to identify four markers associated with resistance, followed by a pathway association study tool (PAST) to identify important metabolic pathways associated with disease severity and incubation period. The ethylene synthesis pathway was significant for disease severity and incubation period. We conducted a greenhouse assay in which we inhibited ethylene to examine the role of ethylene in resistance to NCLB. We observed a significant increase in incubation period and a significant decrease in disease severity between plants treated with the ethylene inhibitor and mock-treated plants. Our study confirms the potential of the BGEM population as a source of novel alleles for resistance. We also confirm the role of ethylene in resistance to NCLB and contribute to the growing body of literature on ethylene and disease resistance in monocots. Frontiers Media S.A. 2023-10-06 /pmc/articles/PMC10587583/ /pubmed/37868313 http://dx.doi.org/10.3389/fpls.2023.1272951 Text en Copyright © 2023 Lipps, Lipka, Mideros and Jamann https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lipps, Sarah
Lipka, Alexander E.
Mideros, Santiago
Jamann, Tiffany
Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population
title Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population
title_full Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population
title_fullStr Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population
title_full_unstemmed Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population
title_short Inhibition of ethylene involved in resistance to E. turcicum in an exotic-derived double haploid maize population
title_sort inhibition of ethylene involved in resistance to e. turcicum in an exotic-derived double haploid maize population
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587583/
https://www.ncbi.nlm.nih.gov/pubmed/37868313
http://dx.doi.org/10.3389/fpls.2023.1272951
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