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A novel sunflower broomrape race with unusual virulence potentially caused by a mutation

INTRODUCTION: The sunflower broomrape (Orobanche cumana Wallr.) gene pools of the Guadalquivir Valley and Cuenca province in Spain had predominantly race-F virulence. A new race G was observed recently in the Guadalquivir Valley potentially due to the genetic recombination of the avirulence genes of...

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Autores principales: Fernández-Melero, Belén, Martín-Sanz, Alberto, del Moral, Lidia, Pérez-Vich, Begoña, Velasco, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587594/
https://www.ncbi.nlm.nih.gov/pubmed/37868306
http://dx.doi.org/10.3389/fpls.2023.1236511
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author Fernández-Melero, Belén
Martín-Sanz, Alberto
del Moral, Lidia
Pérez-Vich, Begoña
Velasco, Leonardo
author_facet Fernández-Melero, Belén
Martín-Sanz, Alberto
del Moral, Lidia
Pérez-Vich, Begoña
Velasco, Leonardo
author_sort Fernández-Melero, Belén
collection PubMed
description INTRODUCTION: The sunflower broomrape (Orobanche cumana Wallr.) gene pools of the Guadalquivir Valley and Cuenca province in Spain had predominantly race-F virulence. A new race G was observed recently in the Guadalquivir Valley potentially due to the genetic recombination of the avirulence genes of both gene pools. METHODS: In this research, we have studied populations with atypical virulence from Cuenca. These populations parasitize on DEB2 sunflower line, resistant to all race-G populations evaluated. Ten populations collected in Cuenca province were evaluated with sunflower differential lines and genotyped with 67 SNP markers. RESULTS: Although genetic recombination with individuals of the Guadalquivir Valley gene pool has been observed in most populations, recombination of avirulence genes was discarded as the cause of the new virulence because the population with the highest degree of attack on DEB2 showed no introgression from an external gene pool. Accordingly, a point mutation is proposed as the putative cause of the new virulence. DISCUSSION: The present study provided a detailed characterization of each population, including the accurate classification of the individuals belonging to each of the classical Spanish gene pools, F1 hybrids, and those that evolved from hybridization between both gene pools. This information is essential to understand how sunflower broomrape populations are evolving in Spain, which in turn may be helpful to understand the dynamics of sunflower broomrape populations in other areas of the world and use this information to develop durable strategies for resistance breeding.
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spelling pubmed-105875942023-10-21 A novel sunflower broomrape race with unusual virulence potentially caused by a mutation Fernández-Melero, Belén Martín-Sanz, Alberto del Moral, Lidia Pérez-Vich, Begoña Velasco, Leonardo Front Plant Sci Plant Science INTRODUCTION: The sunflower broomrape (Orobanche cumana Wallr.) gene pools of the Guadalquivir Valley and Cuenca province in Spain had predominantly race-F virulence. A new race G was observed recently in the Guadalquivir Valley potentially due to the genetic recombination of the avirulence genes of both gene pools. METHODS: In this research, we have studied populations with atypical virulence from Cuenca. These populations parasitize on DEB2 sunflower line, resistant to all race-G populations evaluated. Ten populations collected in Cuenca province were evaluated with sunflower differential lines and genotyped with 67 SNP markers. RESULTS: Although genetic recombination with individuals of the Guadalquivir Valley gene pool has been observed in most populations, recombination of avirulence genes was discarded as the cause of the new virulence because the population with the highest degree of attack on DEB2 showed no introgression from an external gene pool. Accordingly, a point mutation is proposed as the putative cause of the new virulence. DISCUSSION: The present study provided a detailed characterization of each population, including the accurate classification of the individuals belonging to each of the classical Spanish gene pools, F1 hybrids, and those that evolved from hybridization between both gene pools. This information is essential to understand how sunflower broomrape populations are evolving in Spain, which in turn may be helpful to understand the dynamics of sunflower broomrape populations in other areas of the world and use this information to develop durable strategies for resistance breeding. Frontiers Media S.A. 2023-10-06 /pmc/articles/PMC10587594/ /pubmed/37868306 http://dx.doi.org/10.3389/fpls.2023.1236511 Text en Copyright © 2023 Fernández-Melero, Martín-Sanz, del Moral, Pérez-Vich and Velasco https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Fernández-Melero, Belén
Martín-Sanz, Alberto
del Moral, Lidia
Pérez-Vich, Begoña
Velasco, Leonardo
A novel sunflower broomrape race with unusual virulence potentially caused by a mutation
title A novel sunflower broomrape race with unusual virulence potentially caused by a mutation
title_full A novel sunflower broomrape race with unusual virulence potentially caused by a mutation
title_fullStr A novel sunflower broomrape race with unusual virulence potentially caused by a mutation
title_full_unstemmed A novel sunflower broomrape race with unusual virulence potentially caused by a mutation
title_short A novel sunflower broomrape race with unusual virulence potentially caused by a mutation
title_sort novel sunflower broomrape race with unusual virulence potentially caused by a mutation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587594/
https://www.ncbi.nlm.nih.gov/pubmed/37868306
http://dx.doi.org/10.3389/fpls.2023.1236511
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