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Activation of immune evasion machinery is a part of the process of malignant transformation of human cells
Malignant transformation of human cells is associated with their re-programming which results in uncontrolled proliferation and in the same time biochemical activation of immunosuppressive pathways which form cancer immune evasion machinery. However, there is no conceptual understanding of whether i...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587773/ https://www.ncbi.nlm.nih.gov/pubmed/37844478 http://dx.doi.org/10.1016/j.tranon.2023.101805 |
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author | Abooali, Maryam Yasinska, Inna M. Schlichtner, Stephanie Ruggiero, Sabrina Berger, Steffen M. Cholewa, Dietmar Milošević, Milan Bartenstein, Andreas Fasler-Kan, Elizaveta Sumbayev, Vadim V. |
author_facet | Abooali, Maryam Yasinska, Inna M. Schlichtner, Stephanie Ruggiero, Sabrina Berger, Steffen M. Cholewa, Dietmar Milošević, Milan Bartenstein, Andreas Fasler-Kan, Elizaveta Sumbayev, Vadim V. |
author_sort | Abooali, Maryam |
collection | PubMed |
description | Malignant transformation of human cells is associated with their re-programming which results in uncontrolled proliferation and in the same time biochemical activation of immunosuppressive pathways which form cancer immune evasion machinery. However, there is no conceptual understanding of whether immune evasion machinery pathways and expression of immune checkpoint proteins form a part of the process of malignant transformation or if they are triggered by T lymphocytes and natural killers (NK) attempting to attack cells which are undergoing or already underwent malignant transformation. To address this fundamental question, we performed experimental malignant transformation of BEAS-2B human bronchial epithelium cells and RC-124 non-malignant human kidney epithelial cells using bracken extracts containing carcinogenic alkaloid called ptaquiloside. This transformation led to a significant upregulation of cell proliferation velocity and in the same time led to a significant upregulation in expression of key immune checkpoint proteins – galectin-9, programmed death ligand 1 (PD-L1), indoleamine 2,3-dioxygenase (IDO1). Their increased expression levels were in line with upregulation of the levels and activities of HIF-1 transcription complex and transforming growth factor beta type 1 (TGF-β)-Smad3 signalling pathway. When co-cultured with T cells, transformed epithelial cells displayed much higher and more efficient immune evasion activity compared to original non-transformed cells. Therefore, this work resolved a very important scientific and clinical question and suggested that cancer immune evasion machinery is activated during malignant transformation of human cells regardless the presence of immune cells in microenvironment. |
format | Online Article Text |
id | pubmed-10587773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105877732023-10-21 Activation of immune evasion machinery is a part of the process of malignant transformation of human cells Abooali, Maryam Yasinska, Inna M. Schlichtner, Stephanie Ruggiero, Sabrina Berger, Steffen M. Cholewa, Dietmar Milošević, Milan Bartenstein, Andreas Fasler-Kan, Elizaveta Sumbayev, Vadim V. Transl Oncol Commentary Malignant transformation of human cells is associated with their re-programming which results in uncontrolled proliferation and in the same time biochemical activation of immunosuppressive pathways which form cancer immune evasion machinery. However, there is no conceptual understanding of whether immune evasion machinery pathways and expression of immune checkpoint proteins form a part of the process of malignant transformation or if they are triggered by T lymphocytes and natural killers (NK) attempting to attack cells which are undergoing or already underwent malignant transformation. To address this fundamental question, we performed experimental malignant transformation of BEAS-2B human bronchial epithelium cells and RC-124 non-malignant human kidney epithelial cells using bracken extracts containing carcinogenic alkaloid called ptaquiloside. This transformation led to a significant upregulation of cell proliferation velocity and in the same time led to a significant upregulation in expression of key immune checkpoint proteins – galectin-9, programmed death ligand 1 (PD-L1), indoleamine 2,3-dioxygenase (IDO1). Their increased expression levels were in line with upregulation of the levels and activities of HIF-1 transcription complex and transforming growth factor beta type 1 (TGF-β)-Smad3 signalling pathway. When co-cultured with T cells, transformed epithelial cells displayed much higher and more efficient immune evasion activity compared to original non-transformed cells. Therefore, this work resolved a very important scientific and clinical question and suggested that cancer immune evasion machinery is activated during malignant transformation of human cells regardless the presence of immune cells in microenvironment. Neoplasia Press 2023-10-14 /pmc/articles/PMC10587773/ /pubmed/37844478 http://dx.doi.org/10.1016/j.tranon.2023.101805 Text en © 2023 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Commentary Abooali, Maryam Yasinska, Inna M. Schlichtner, Stephanie Ruggiero, Sabrina Berger, Steffen M. Cholewa, Dietmar Milošević, Milan Bartenstein, Andreas Fasler-Kan, Elizaveta Sumbayev, Vadim V. Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
title | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
title_full | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
title_fullStr | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
title_full_unstemmed | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
title_short | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
title_sort | activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
topic | Commentary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587773/ https://www.ncbi.nlm.nih.gov/pubmed/37844478 http://dx.doi.org/10.1016/j.tranon.2023.101805 |
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