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Controlling the interaction between CaMKII and Calmodulin with a photocrosslinking unnatural amino acid

Using unnatural amino acid mutagenesis, we made a mutant of CaMKII that forms a covalent linkage to Calmodulin upon illumination by UV light. Like wild‐type CaMKII, the L308BzF mutant stoichiometrically binds to Calmodulin, in a calcium‐dependent manner. Using this construct, we demonstrate that Cal...

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Detalles Bibliográficos
Autores principales:	Lučić, Iva, Jiang, Pin‐Lian, Franz, Andreas, Bursztyn, Yuval, Liu, Fan, Plested, Andrew J. R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley & Sons, Inc. 2023
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588329/ https://www.ncbi.nlm.nih.gov/pubmed/37784242 http://dx.doi.org/10.1002/pro.4798

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