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HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis
BACKGROUND: Allergic rhinitis (AR) is a common nasal inflammatory disorder that severely affects an individual's quality of life (QoL) and poses a heavy financial burden. In addition to routine treatments, probiotic intervention has emerged as a promising strategy for preventing and alleviating...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588638/ https://www.ncbi.nlm.nih.gov/pubmed/37869664 http://dx.doi.org/10.3389/fmicb.2023.1202858 |
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author | Li, Lisha Wen, Xueyi Gong, Yiyi Chen, Yuling Xu, Jiatong Sun, Jinlyu Deng, Haiteng Guan, Kai |
author_facet | Li, Lisha Wen, Xueyi Gong, Yiyi Chen, Yuling Xu, Jiatong Sun, Jinlyu Deng, Haiteng Guan, Kai |
author_sort | Li, Lisha |
collection | PubMed |
description | BACKGROUND: Allergic rhinitis (AR) is a common nasal inflammatory disorder that severely affects an individual's quality of life (QoL) and poses a heavy financial burden. In addition to routine treatments, probiotic intervention has emerged as a promising strategy for preventing and alleviating allergic diseases. The main objective of this study was to determine the effect of a novel multi-strain probiotic mixture on AR symptoms and investigate potential targets underlying the probiotic intervention. METHODS: A randomized, double-blind, placebo-controlled clinical study was conducted on AR patients who were allergic to autumnal pollens (n = 31). Placebo or a novel probiotic mixture, composed of Lactobacillus rhamnosus (L. rhamnosus) HN001, L. acidophilus NCFM, Bifidobacterium lactis (B. lactis) Bi-07, L. paracasei LPC-37, and L. reuteri LE16, was administered after 2 months. The therapeutic efficacy was evaluated by a symptom assessment scale. Before and during the pollen season, blood samples were collected, and peripheral blood mononuclear cells (PBMCs) were isolated for further tandem mass tags (TMTs)-based quantitative proteomic analyses. Potential targets and underlying pathological pathways were explored using bioinformatics methods. RESULTS: During the pollen season, the rhinoconjunctivitis symptom score of participants who were administered probiotics (probiotic group, n = 15) was significantly lower than those administered placebo (placebo group, n = 15) (P = 0.037). The proteomic analyses identified 60 differentially expressed proteins (DEPs) in the placebo group, and subsequent enrichment analyses enriched a series of pathways and biological processes, including signaling pathways of inflammation, coagulation cascade, lipid, carbohydrate and amino acid metabolic pathways, and transcription and translation processes. Least Absolute Shrinkage and Selection Operator (LASSO) regression extracted five main elements, namely, GSTO1, ATP2A2, MCM7, PROS1, and TRIM58, as signature proteins. A total of 17 DEPs were identified in the probiotic group, and there was no pathway enriched. Comparison of DEPs in the two groups revealed that the expression levels of the high-mobility group nucleosome-binding domain-containing protein 2 (HMGN2) and Histone H1.2 presented an opposite trend with different interventions. CONCLUSION: Our data showed that AR symptoms alleviated after treatment with the novel multi-strain probiotic mixture, and the proteomic analyses suggested that HMGN2 and Histone H1.2 might be targets of probiotic intervention for seasonal AR. |
format | Online Article Text |
id | pubmed-10588638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105886382023-10-21 HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis Li, Lisha Wen, Xueyi Gong, Yiyi Chen, Yuling Xu, Jiatong Sun, Jinlyu Deng, Haiteng Guan, Kai Front Microbiol Microbiology BACKGROUND: Allergic rhinitis (AR) is a common nasal inflammatory disorder that severely affects an individual's quality of life (QoL) and poses a heavy financial burden. In addition to routine treatments, probiotic intervention has emerged as a promising strategy for preventing and alleviating allergic diseases. The main objective of this study was to determine the effect of a novel multi-strain probiotic mixture on AR symptoms and investigate potential targets underlying the probiotic intervention. METHODS: A randomized, double-blind, placebo-controlled clinical study was conducted on AR patients who were allergic to autumnal pollens (n = 31). Placebo or a novel probiotic mixture, composed of Lactobacillus rhamnosus (L. rhamnosus) HN001, L. acidophilus NCFM, Bifidobacterium lactis (B. lactis) Bi-07, L. paracasei LPC-37, and L. reuteri LE16, was administered after 2 months. The therapeutic efficacy was evaluated by a symptom assessment scale. Before and during the pollen season, blood samples were collected, and peripheral blood mononuclear cells (PBMCs) were isolated for further tandem mass tags (TMTs)-based quantitative proteomic analyses. Potential targets and underlying pathological pathways were explored using bioinformatics methods. RESULTS: During the pollen season, the rhinoconjunctivitis symptom score of participants who were administered probiotics (probiotic group, n = 15) was significantly lower than those administered placebo (placebo group, n = 15) (P = 0.037). The proteomic analyses identified 60 differentially expressed proteins (DEPs) in the placebo group, and subsequent enrichment analyses enriched a series of pathways and biological processes, including signaling pathways of inflammation, coagulation cascade, lipid, carbohydrate and amino acid metabolic pathways, and transcription and translation processes. Least Absolute Shrinkage and Selection Operator (LASSO) regression extracted five main elements, namely, GSTO1, ATP2A2, MCM7, PROS1, and TRIM58, as signature proteins. A total of 17 DEPs were identified in the probiotic group, and there was no pathway enriched. Comparison of DEPs in the two groups revealed that the expression levels of the high-mobility group nucleosome-binding domain-containing protein 2 (HMGN2) and Histone H1.2 presented an opposite trend with different interventions. CONCLUSION: Our data showed that AR symptoms alleviated after treatment with the novel multi-strain probiotic mixture, and the proteomic analyses suggested that HMGN2 and Histone H1.2 might be targets of probiotic intervention for seasonal AR. Frontiers Media S.A. 2023-10-06 /pmc/articles/PMC10588638/ /pubmed/37869664 http://dx.doi.org/10.3389/fmicb.2023.1202858 Text en Copyright © 2023 Li, Wen, Gong, Chen, Xu, Sun, Deng and Guan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Lisha Wen, Xueyi Gong, Yiyi Chen, Yuling Xu, Jiatong Sun, Jinlyu Deng, Haiteng Guan, Kai HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
title | HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
title_full | HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
title_fullStr | HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
title_full_unstemmed | HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
title_short | HMGN2 and Histone H1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
title_sort | hmgn2 and histone h1.2: potential targets of a novel probiotic mixture for seasonal allergic rhinitis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588638/ https://www.ncbi.nlm.nih.gov/pubmed/37869664 http://dx.doi.org/10.3389/fmicb.2023.1202858 |
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