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Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy

Clinical identification and fundamental study of viruses rely on the detection of viral proteins or viral nucleic acids. Yet, amplification-based and antigen-based methods are not able to provide precise compositional information of individual virions due to small particle size and low-abundance che...

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Autores principales: Xia, Qing, Guo, Zhongyue, Zong, Haonan, Seitz, Scott, Yurdakul, Celalettin, Ünlü, M. Selim, Wang, Le, Connor, John H., Cheng, Ji-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589364/
https://www.ncbi.nlm.nih.gov/pubmed/37863905
http://dx.doi.org/10.1038/s41467-023-42439-4
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author Xia, Qing
Guo, Zhongyue
Zong, Haonan
Seitz, Scott
Yurdakul, Celalettin
Ünlü, M. Selim
Wang, Le
Connor, John H.
Cheng, Ji-Xin
author_facet Xia, Qing
Guo, Zhongyue
Zong, Haonan
Seitz, Scott
Yurdakul, Celalettin
Ünlü, M. Selim
Wang, Le
Connor, John H.
Cheng, Ji-Xin
author_sort Xia, Qing
collection PubMed
description Clinical identification and fundamental study of viruses rely on the detection of viral proteins or viral nucleic acids. Yet, amplification-based and antigen-based methods are not able to provide precise compositional information of individual virions due to small particle size and low-abundance chemical contents (e.g., ~ 5000 proteins in a vesicular stomatitis virus). Here, we report a widefield interferometric defocus-enhanced mid-infrared photothermal (WIDE-MIP) microscope for high-throughput fingerprinting of single viruses. With the identification of feature absorption peaks, WIDE-MIP reveals the contents of viral proteins and nucleic acids in single DNA vaccinia viruses and RNA vesicular stomatitis viruses. Different nucleic acid signatures of thymine and uracil residue vibrations are obtained to differentiate DNA and RNA viruses. WIDE-MIP imaging further reveals an enriched β sheet components in DNA varicella-zoster virus proteins. Together, these advances open a new avenue for compositional analysis of viral vectors and elucidating protein function in an assembled virion.
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spelling pubmed-105893642023-10-22 Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy Xia, Qing Guo, Zhongyue Zong, Haonan Seitz, Scott Yurdakul, Celalettin Ünlü, M. Selim Wang, Le Connor, John H. Cheng, Ji-Xin Nat Commun Article Clinical identification and fundamental study of viruses rely on the detection of viral proteins or viral nucleic acids. Yet, amplification-based and antigen-based methods are not able to provide precise compositional information of individual virions due to small particle size and low-abundance chemical contents (e.g., ~ 5000 proteins in a vesicular stomatitis virus). Here, we report a widefield interferometric defocus-enhanced mid-infrared photothermal (WIDE-MIP) microscope for high-throughput fingerprinting of single viruses. With the identification of feature absorption peaks, WIDE-MIP reveals the contents of viral proteins and nucleic acids in single DNA vaccinia viruses and RNA vesicular stomatitis viruses. Different nucleic acid signatures of thymine and uracil residue vibrations are obtained to differentiate DNA and RNA viruses. WIDE-MIP imaging further reveals an enriched β sheet components in DNA varicella-zoster virus proteins. Together, these advances open a new avenue for compositional analysis of viral vectors and elucidating protein function in an assembled virion. Nature Publishing Group UK 2023-10-20 /pmc/articles/PMC10589364/ /pubmed/37863905 http://dx.doi.org/10.1038/s41467-023-42439-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Xia, Qing
Guo, Zhongyue
Zong, Haonan
Seitz, Scott
Yurdakul, Celalettin
Ünlü, M. Selim
Wang, Le
Connor, John H.
Cheng, Ji-Xin
Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
title Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
title_full Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
title_fullStr Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
title_full_unstemmed Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
title_short Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
title_sort single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589364/
https://www.ncbi.nlm.nih.gov/pubmed/37863905
http://dx.doi.org/10.1038/s41467-023-42439-4
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