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Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics

As the major channel of aqueous humor outflow, dysfunction of trabecular meshwork (TM) can lead to intraocular pressure elevating, which can trigger primary open-angle glaucoma (POAG). In this study, we use single-cell RNA sequencing (scRNA-seq) technique to build an atlas and further explore the sp...

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Autores principales: Jia, Xu, Wu, Jian, Chen, Xiaohong, Hou, Simeng, Li, Yangyang, Zhao, Ling, Zhu, Yingting, Li, Zhidong, Deng, Caibin, Su, Wenru, Zhuo, Yehong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589847/
https://www.ncbi.nlm.nih.gov/pubmed/37867950
http://dx.doi.org/10.1016/j.isci.2023.108024
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author Jia, Xu
Wu, Jian
Chen, Xiaohong
Hou, Simeng
Li, Yangyang
Zhao, Ling
Zhu, Yingting
Li, Zhidong
Deng, Caibin
Su, Wenru
Zhuo, Yehong
author_facet Jia, Xu
Wu, Jian
Chen, Xiaohong
Hou, Simeng
Li, Yangyang
Zhao, Ling
Zhu, Yingting
Li, Zhidong
Deng, Caibin
Su, Wenru
Zhuo, Yehong
author_sort Jia, Xu
collection PubMed
description As the major channel of aqueous humor outflow, dysfunction of trabecular meshwork (TM) can lead to intraocular pressure elevating, which can trigger primary open-angle glaucoma (POAG). In this study, we use single-cell RNA sequencing (scRNA-seq) technique to build an atlas and further explore the spontaneous POAG and healthy macaques cellular heterogeneity associated with the dysfunction of TM contraction. We built the TM atlas, which identified 14 different cell types. In Beam A, Beam B, Beam C, and smooth muscle cell (SMC) cell types, we first found multiple genes associated with TM contraction (e.g., TPM1, ACTC1, TNNT1), determining their differential expression in the POAG and healthy groups. In addition, the microstructural alterations in TM of POAG non-human primates were observed, which was compact and collapsed. Thus, our study indicated that TPM1 may be a key target for regulating TM structure, contraction function, and resistance of aqueous humor outflow.
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spelling pubmed-105898472023-10-22 Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics Jia, Xu Wu, Jian Chen, Xiaohong Hou, Simeng Li, Yangyang Zhao, Ling Zhu, Yingting Li, Zhidong Deng, Caibin Su, Wenru Zhuo, Yehong iScience Article As the major channel of aqueous humor outflow, dysfunction of trabecular meshwork (TM) can lead to intraocular pressure elevating, which can trigger primary open-angle glaucoma (POAG). In this study, we use single-cell RNA sequencing (scRNA-seq) technique to build an atlas and further explore the spontaneous POAG and healthy macaques cellular heterogeneity associated with the dysfunction of TM contraction. We built the TM atlas, which identified 14 different cell types. In Beam A, Beam B, Beam C, and smooth muscle cell (SMC) cell types, we first found multiple genes associated with TM contraction (e.g., TPM1, ACTC1, TNNT1), determining their differential expression in the POAG and healthy groups. In addition, the microstructural alterations in TM of POAG non-human primates were observed, which was compact and collapsed. Thus, our study indicated that TPM1 may be a key target for regulating TM structure, contraction function, and resistance of aqueous humor outflow. Elsevier 2023-09-22 /pmc/articles/PMC10589847/ /pubmed/37867950 http://dx.doi.org/10.1016/j.isci.2023.108024 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Jia, Xu
Wu, Jian
Chen, Xiaohong
Hou, Simeng
Li, Yangyang
Zhao, Ling
Zhu, Yingting
Li, Zhidong
Deng, Caibin
Su, Wenru
Zhuo, Yehong
Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics
title Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics
title_full Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics
title_fullStr Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics
title_full_unstemmed Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics
title_short Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics
title_sort cell atlas of trabecular meshwork in glaucomatous non-human primates and degs related to tissue contract based on single-cell transcriptomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589847/
https://www.ncbi.nlm.nih.gov/pubmed/37867950
http://dx.doi.org/10.1016/j.isci.2023.108024
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