Protocol for rapidly inducing genome-wide RNA Pol II hyperphosphorylation by selectively disrupting INTAC phosphatase activity

While several inhibitors targeting RNA polymerase II (Pol II) kinases have been applied for inhibiting RNA Pol II phosphorylation, there are few approaches for inducing RNA Pol II hyperphosphorylation. Here, we present a protocol for constructing the INTS8 degradation tag (dTAG) system combined with...

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Detalles Bibliográficos
Autores principales: Ji, Yu-Xin, Hu, Shibin, Chen, Fei Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589876/
https://www.ncbi.nlm.nih.gov/pubmed/37831607
http://dx.doi.org/10.1016/j.xpro.2023.102640

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