Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in develo...

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Detalles Bibliográficos
Autores principales: Hageter, John, Starkey, Jacob, Barr, Allison, Huff, Johnathon R., Horstick, Eric J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589883/
https://www.ncbi.nlm.nih.gov/pubmed/37837624
http://dx.doi.org/10.1016/j.xpro.2023.102636
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author Hageter, John
Starkey, Jacob
Barr, Allison
Huff, Johnathon R.
Horstick, Eric J.
author_facet Hageter, John
Starkey, Jacob
Barr, Allison
Huff, Johnathon R.
Horstick, Eric J.
author_sort Hageter, John
collection PubMed
description Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity. For complete details on the use and execution of this protocol, please refer to Hageter et al. (2023).(1)
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spelling pubmed-105898832023-10-22 Protocol for controlling visual experience during zebrafish development and modulation of motor behavior Hageter, John Starkey, Jacob Barr, Allison Huff, Johnathon R. Horstick, Eric J. STAR Protoc Protocol Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity. For complete details on the use and execution of this protocol, please refer to Hageter et al. (2023).(1) Elsevier 2023-10-13 /pmc/articles/PMC10589883/ /pubmed/37837624 http://dx.doi.org/10.1016/j.xpro.2023.102636 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hageter, John
Starkey, Jacob
Barr, Allison
Huff, Johnathon R.
Horstick, Eric J.
Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
title Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
title_full Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
title_fullStr Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
title_full_unstemmed Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
title_short Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
title_sort protocol for controlling visual experience during zebrafish development and modulation of motor behavior
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589883/
https://www.ncbi.nlm.nih.gov/pubmed/37837624
http://dx.doi.org/10.1016/j.xpro.2023.102636
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