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Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease

Cause of Kawasaki disease (KD) is unknown. KD is often resistant to treatment with intravenous immunoglobulin (IVIG). Sano’s score, which is derived from total bilirubin (TBIL), aspartate aminotransferase (AST) and C-reactive protein (CRP), is predictive of IVIG resistance in Japan. A recent study r...

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Autores principales: Spezia, Pietro Giorgio, Matsudaira, Kazunari, Filippini, Fabio, Miyamura, Takako, Okada, Keiko, Nagao, Yoshiro, Ishida, Takafumi, Sano, Tetsuya, Pistello, Mauro, Maggi, Fabrizio, Hara, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590372/
https://www.ncbi.nlm.nih.gov/pubmed/37865714
http://dx.doi.org/10.1038/s41598-023-45327-5
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author Spezia, Pietro Giorgio
Matsudaira, Kazunari
Filippini, Fabio
Miyamura, Takako
Okada, Keiko
Nagao, Yoshiro
Ishida, Takafumi
Sano, Tetsuya
Pistello, Mauro
Maggi, Fabrizio
Hara, Junichi
author_facet Spezia, Pietro Giorgio
Matsudaira, Kazunari
Filippini, Fabio
Miyamura, Takako
Okada, Keiko
Nagao, Yoshiro
Ishida, Takafumi
Sano, Tetsuya
Pistello, Mauro
Maggi, Fabrizio
Hara, Junichi
author_sort Spezia, Pietro Giorgio
collection PubMed
description Cause of Kawasaki disease (KD) is unknown. KD is often resistant to treatment with intravenous immunoglobulin (IVIG). Sano’s score, which is derived from total bilirubin (TBIL), aspartate aminotransferase (AST) and C-reactive protein (CRP), is predictive of IVIG resistance in Japan. A recent study reported that Torquetenovirus (TTV), especially TTV7, was present at a high viral load in the patients with KD. We used PCR to quantify TTV load and amplicon next generation sequencing to detect individual TTV species. We used serum samples that were collected between 2002 and 2005 from 57 Japanese KD patients before IVIG treatment. Correlations between TTV load and Sano’s score, the biomarkers that constitute this score, and IVIG resistance were examined. TTV load was positively correlated with Sano’s score (P = 0.0248), TBIL (P = 0.0004), and AST (P = 0.0385), but not with CRP (P = 0.6178). TTV load was marginally correlated with IVIG resistance (P = 0.1544). Presence of TTV7 was correlated with total TTV load significantly (P = 0.0231). The correlations between biomarkers for KD and TTV load suggested that TTV may play a role in the pathophysiology of KD. We hypothesize that TTV7 may be associated with a higher total viral load in KD.
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spelling pubmed-105903722023-10-23 Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease Spezia, Pietro Giorgio Matsudaira, Kazunari Filippini, Fabio Miyamura, Takako Okada, Keiko Nagao, Yoshiro Ishida, Takafumi Sano, Tetsuya Pistello, Mauro Maggi, Fabrizio Hara, Junichi Sci Rep Article Cause of Kawasaki disease (KD) is unknown. KD is often resistant to treatment with intravenous immunoglobulin (IVIG). Sano’s score, which is derived from total bilirubin (TBIL), aspartate aminotransferase (AST) and C-reactive protein (CRP), is predictive of IVIG resistance in Japan. A recent study reported that Torquetenovirus (TTV), especially TTV7, was present at a high viral load in the patients with KD. We used PCR to quantify TTV load and amplicon next generation sequencing to detect individual TTV species. We used serum samples that were collected between 2002 and 2005 from 57 Japanese KD patients before IVIG treatment. Correlations between TTV load and Sano’s score, the biomarkers that constitute this score, and IVIG resistance were examined. TTV load was positively correlated with Sano’s score (P = 0.0248), TBIL (P = 0.0004), and AST (P = 0.0385), but not with CRP (P = 0.6178). TTV load was marginally correlated with IVIG resistance (P = 0.1544). Presence of TTV7 was correlated with total TTV load significantly (P = 0.0231). The correlations between biomarkers for KD and TTV load suggested that TTV may play a role in the pathophysiology of KD. We hypothesize that TTV7 may be associated with a higher total viral load in KD. Nature Publishing Group UK 2023-10-21 /pmc/articles/PMC10590372/ /pubmed/37865714 http://dx.doi.org/10.1038/s41598-023-45327-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Spezia, Pietro Giorgio
Matsudaira, Kazunari
Filippini, Fabio
Miyamura, Takako
Okada, Keiko
Nagao, Yoshiro
Ishida, Takafumi
Sano, Tetsuya
Pistello, Mauro
Maggi, Fabrizio
Hara, Junichi
Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease
title Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease
title_full Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease
title_fullStr Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease
title_full_unstemmed Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease
title_short Viral load of Torquetenovirus correlates with Sano’s score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease
title_sort viral load of torquetenovirus correlates with sano’s score and levels of total bilirubin and aspartate aminotransferase in kawasaki disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590372/
https://www.ncbi.nlm.nih.gov/pubmed/37865714
http://dx.doi.org/10.1038/s41598-023-45327-5
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