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Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice
The importance of sexual dimorphism has been highlighted in recent years since the National Institutes of Health's mandate on considering sex as a biological variable. Although recent studies have taken strides to study both sexes side by side, investigations into the normal physiological diffe...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590649/ https://www.ncbi.nlm.nih.gov/pubmed/37873500 http://dx.doi.org/10.1210/jendso/bvad099 |
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author | Jo, Seokwon Beetch, Megan Gustafson, Eric Wong, Alicia Oribamise, Eunice Chung, Grace Vadrevu, Suryakiran Satin, Leslie S Bernal-Mizrachi, Ernesto Alejandro, Emilyn U |
author_facet | Jo, Seokwon Beetch, Megan Gustafson, Eric Wong, Alicia Oribamise, Eunice Chung, Grace Vadrevu, Suryakiran Satin, Leslie S Bernal-Mizrachi, Ernesto Alejandro, Emilyn U |
author_sort | Jo, Seokwon |
collection | PubMed |
description | The importance of sexual dimorphism has been highlighted in recent years since the National Institutes of Health's mandate on considering sex as a biological variable. Although recent studies have taken strides to study both sexes side by side, investigations into the normal physiological differences between males and females are limited. In this study, we aimed to characterized sex-dependent differences in glucose metabolism and pancreatic β-cell physiology in normal conditions using C57BL/6J mice, the most common mouse strain used in metabolic studies. Here, we report that female mice have improved glucose and insulin tolerance associated with lower nonfasted blood glucose and insulin levels compared with male mice at 3 and 6 months of age. Both male and female animals show β-cell mass expansion from embryonic day 17.5 to adulthood, and no sex differences were observed at embryonic day 17.5, newborn, 1 month, or 3 months of age. However, 6-month-old males displayed increased β-cell mass in response to insulin resistance compared with littermate females. Molecularly, we uncovered sexual dimorphic alterations in the protein levels of nutrient sensing proteins O-GlcNAc transferase and mTOR, as well as differences in glucose-stimulus coupling mechanisms that may underlie the differences in sexually dimorphic β-cell physiology observed in C57BL/6J mice. |
format | Online Article Text |
id | pubmed-10590649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105906492023-10-23 Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice Jo, Seokwon Beetch, Megan Gustafson, Eric Wong, Alicia Oribamise, Eunice Chung, Grace Vadrevu, Suryakiran Satin, Leslie S Bernal-Mizrachi, Ernesto Alejandro, Emilyn U J Endocr Soc Research Article The importance of sexual dimorphism has been highlighted in recent years since the National Institutes of Health's mandate on considering sex as a biological variable. Although recent studies have taken strides to study both sexes side by side, investigations into the normal physiological differences between males and females are limited. In this study, we aimed to characterized sex-dependent differences in glucose metabolism and pancreatic β-cell physiology in normal conditions using C57BL/6J mice, the most common mouse strain used in metabolic studies. Here, we report that female mice have improved glucose and insulin tolerance associated with lower nonfasted blood glucose and insulin levels compared with male mice at 3 and 6 months of age. Both male and female animals show β-cell mass expansion from embryonic day 17.5 to adulthood, and no sex differences were observed at embryonic day 17.5, newborn, 1 month, or 3 months of age. However, 6-month-old males displayed increased β-cell mass in response to insulin resistance compared with littermate females. Molecularly, we uncovered sexual dimorphic alterations in the protein levels of nutrient sensing proteins O-GlcNAc transferase and mTOR, as well as differences in glucose-stimulus coupling mechanisms that may underlie the differences in sexually dimorphic β-cell physiology observed in C57BL/6J mice. Oxford University Press 2023-07-25 /pmc/articles/PMC10590649/ /pubmed/37873500 http://dx.doi.org/10.1210/jendso/bvad099 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Jo, Seokwon Beetch, Megan Gustafson, Eric Wong, Alicia Oribamise, Eunice Chung, Grace Vadrevu, Suryakiran Satin, Leslie S Bernal-Mizrachi, Ernesto Alejandro, Emilyn U Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice |
title | Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice |
title_full | Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice |
title_fullStr | Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice |
title_full_unstemmed | Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice |
title_short | Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice |
title_sort | sex differences in pancreatic β-cell physiology and glucose homeostasis in c57bl/6j mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590649/ https://www.ncbi.nlm.nih.gov/pubmed/37873500 http://dx.doi.org/10.1210/jendso/bvad099 |
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