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Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines

Nitrogen (N) limits crop production, yet more than half of N fertilizer inputs are lost to the environment. Developing maize hybrids with improved N use efficiency can help minimize N losses and in turn reduce adverse ecological, economical, and health consequences. This study aimed to identify sing...

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Autores principales: Sanchez, Darlene L., Santana, Alice Silva, Morais, Palloma Indiara Caproni, Peterlini, Edicarlos, De La Fuente, Gerald, Castellano, Michael J., Blanco, Michael, Lübberstedt, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590880/
https://www.ncbi.nlm.nih.gov/pubmed/37877090
http://dx.doi.org/10.3389/fpls.2023.1270166
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author Sanchez, Darlene L.
Santana, Alice Silva
Morais, Palloma Indiara Caproni
Peterlini, Edicarlos
De La Fuente, Gerald
Castellano, Michael J.
Blanco, Michael
Lübberstedt, Thomas
author_facet Sanchez, Darlene L.
Santana, Alice Silva
Morais, Palloma Indiara Caproni
Peterlini, Edicarlos
De La Fuente, Gerald
Castellano, Michael J.
Blanco, Michael
Lübberstedt, Thomas
author_sort Sanchez, Darlene L.
collection PubMed
description Nitrogen (N) limits crop production, yet more than half of N fertilizer inputs are lost to the environment. Developing maize hybrids with improved N use efficiency can help minimize N losses and in turn reduce adverse ecological, economical, and health consequences. This study aimed to identify single nucleotide polymorphisms (SNPs) associated with agronomic traits (plant height, grain yield, and anthesis to silking interval) under high and low N conditions. A genome-wide association study (GWAS) was conducted using 181 doubled haploid (DH) lines derived from crosses between landraces from the Germplasm Enhancement of Maize (BGEM lines) project and two inbreds, PHB47 and PHZ51. These DH lines were genotyped using 62,077 SNP markers. The same lines from the per se trials were used as parental lines for the testcross field trials. Plant height, anthesis to silking interval, and grain yield were collected from high and low N conditions in three environments for both per se and testcross trials. We used three GWAS models, namely, general linear model (GLM), mixed linear model (MLM), and Fixed and Random model Circulating Probability Unification (FarmCPU) model. We observed significant genetic variation among the DH lines and their derived testcrosses. Interestingly, some testcrosses of exotic introgression lines were superior under high and low N conditions compared to the check hybrid, PHB47/PHZ51. We detected multiple SNPs associated with agronomic traits under high and low N, some of which co-localized with gene models associated with stress response and N metabolism. The BGEM panel is, thus, a promising source of allelic diversity for genes controlling agronomic traits under different N conditions.
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spelling pubmed-105908802023-10-24 Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines Sanchez, Darlene L. Santana, Alice Silva Morais, Palloma Indiara Caproni Peterlini, Edicarlos De La Fuente, Gerald Castellano, Michael J. Blanco, Michael Lübberstedt, Thomas Front Plant Sci Plant Science Nitrogen (N) limits crop production, yet more than half of N fertilizer inputs are lost to the environment. Developing maize hybrids with improved N use efficiency can help minimize N losses and in turn reduce adverse ecological, economical, and health consequences. This study aimed to identify single nucleotide polymorphisms (SNPs) associated with agronomic traits (plant height, grain yield, and anthesis to silking interval) under high and low N conditions. A genome-wide association study (GWAS) was conducted using 181 doubled haploid (DH) lines derived from crosses between landraces from the Germplasm Enhancement of Maize (BGEM lines) project and two inbreds, PHB47 and PHZ51. These DH lines were genotyped using 62,077 SNP markers. The same lines from the per se trials were used as parental lines for the testcross field trials. Plant height, anthesis to silking interval, and grain yield were collected from high and low N conditions in three environments for both per se and testcross trials. We used three GWAS models, namely, general linear model (GLM), mixed linear model (MLM), and Fixed and Random model Circulating Probability Unification (FarmCPU) model. We observed significant genetic variation among the DH lines and their derived testcrosses. Interestingly, some testcrosses of exotic introgression lines were superior under high and low N conditions compared to the check hybrid, PHB47/PHZ51. We detected multiple SNPs associated with agronomic traits under high and low N, some of which co-localized with gene models associated with stress response and N metabolism. The BGEM panel is, thus, a promising source of allelic diversity for genes controlling agronomic traits under different N conditions. Frontiers Media S.A. 2023-10-09 /pmc/articles/PMC10590880/ /pubmed/37877090 http://dx.doi.org/10.3389/fpls.2023.1270166 Text en Copyright © 2023 Sanchez, Santana, Morais, Peterlini, De La Fuente, Castellano, Blanco and Lübberstedt https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sanchez, Darlene L.
Santana, Alice Silva
Morais, Palloma Indiara Caproni
Peterlini, Edicarlos
De La Fuente, Gerald
Castellano, Michael J.
Blanco, Michael
Lübberstedt, Thomas
Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines
title Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines
title_full Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines
title_fullStr Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines
title_full_unstemmed Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines
title_short Phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (Zea mays L.) exotic introgression lines
title_sort phenotypic and genome-wide association analyses for nitrogen use efficiency related traits in maize (zea mays l.) exotic introgression lines
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590880/
https://www.ncbi.nlm.nih.gov/pubmed/37877090
http://dx.doi.org/10.3389/fpls.2023.1270166
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