Cargando…

High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner

INTRODUCTION: Obesity consists in the accumulation of adipose tissue accompanied by low grade chronic inflammation and is considered a pandemic disease. Recent studies have observed that obesity affects females and males in a sex-dependent manner. In addition, several works have demonstrated that pa...

Descripción completa

Detalles Bibliográficos
Autores principales: Escalona, Rene, Larqué, Carlos, Cortes, Daniela, Vilchis, Ricardo, Granados-Delgado, Emiliano, Sánchez, Abigail, Sánchez-Bringas, Guadalupe, Lugo-Martínez, Haydée
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591070/
https://www.ncbi.nlm.nih.gov/pubmed/37876538
http://dx.doi.org/10.3389/fendo.2023.1246194
_version_ 1785124145172840448
author Escalona, Rene
Larqué, Carlos
Cortes, Daniela
Vilchis, Ricardo
Granados-Delgado, Emiliano
Sánchez, Abigail
Sánchez-Bringas, Guadalupe
Lugo-Martínez, Haydée
author_facet Escalona, Rene
Larqué, Carlos
Cortes, Daniela
Vilchis, Ricardo
Granados-Delgado, Emiliano
Sánchez, Abigail
Sánchez-Bringas, Guadalupe
Lugo-Martínez, Haydée
author_sort Escalona, Rene
collection PubMed
description INTRODUCTION: Obesity consists in the accumulation of adipose tissue accompanied by low grade chronic inflammation and is considered a pandemic disease. Recent studies have observed that obesity affects females and males in a sex-dependent manner. In addition, several works have demonstrated that parental obesity increases the risk to develop obesity, insulin resistance, diabetes, and reproductive disorders. Considering that intergenerational effects of obesity may occur in a sex-dependent manner, we studied male Wistar rat progeny (F1) obtained from mothers or fathers (F0) fed on a high-fat diet (HFD). METHODS: Five-week-old female and male Wistar rats were fed on a HFD (with 60% of calories provided by fat) for 18 weeks (F0). At the end of the treatment, animals were mated with young rats to obtain their progeny (F1). After weaning, F1 animals were fed on standard chow until 18 weeks of age. Body weight gain, fasting plasma glucose, insulin and leptin levels, glucose tolerance, insulin sensitivity, and adiposity were evaluated. In addition, beta-cell expression of nuclear p16 was assessed by immunofluorescence. RESULTS AND CONCLUSIONS: HFD altered plasma fasting glucose, insulin and leptin levels, glucose tolerance, adiposity, and beta-cell expression of p16 in F0 rats. Particularly, HFD showed sexual dimorphic effects on body weight gain and insulin sensitivity. Moreover, we observed that parental HFD feeding exerts parental-sex-specific metabolic impairment in the male progeny. Finally, parental metabolic dysfunction could be in part attributed to the increased beta-cell expression of p16; other mechanisms could be involved in the offspring glucose homeostasis.
format Online
Article
Text
id pubmed-10591070
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105910702023-10-24 High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner Escalona, Rene Larqué, Carlos Cortes, Daniela Vilchis, Ricardo Granados-Delgado, Emiliano Sánchez, Abigail Sánchez-Bringas, Guadalupe Lugo-Martínez, Haydée Front Endocrinol (Lausanne) Endocrinology INTRODUCTION: Obesity consists in the accumulation of adipose tissue accompanied by low grade chronic inflammation and is considered a pandemic disease. Recent studies have observed that obesity affects females and males in a sex-dependent manner. In addition, several works have demonstrated that parental obesity increases the risk to develop obesity, insulin resistance, diabetes, and reproductive disorders. Considering that intergenerational effects of obesity may occur in a sex-dependent manner, we studied male Wistar rat progeny (F1) obtained from mothers or fathers (F0) fed on a high-fat diet (HFD). METHODS: Five-week-old female and male Wistar rats were fed on a HFD (with 60% of calories provided by fat) for 18 weeks (F0). At the end of the treatment, animals were mated with young rats to obtain their progeny (F1). After weaning, F1 animals were fed on standard chow until 18 weeks of age. Body weight gain, fasting plasma glucose, insulin and leptin levels, glucose tolerance, insulin sensitivity, and adiposity were evaluated. In addition, beta-cell expression of nuclear p16 was assessed by immunofluorescence. RESULTS AND CONCLUSIONS: HFD altered plasma fasting glucose, insulin and leptin levels, glucose tolerance, adiposity, and beta-cell expression of p16 in F0 rats. Particularly, HFD showed sexual dimorphic effects on body weight gain and insulin sensitivity. Moreover, we observed that parental HFD feeding exerts parental-sex-specific metabolic impairment in the male progeny. Finally, parental metabolic dysfunction could be in part attributed to the increased beta-cell expression of p16; other mechanisms could be involved in the offspring glucose homeostasis. Frontiers Media S.A. 2023-10-09 /pmc/articles/PMC10591070/ /pubmed/37876538 http://dx.doi.org/10.3389/fendo.2023.1246194 Text en Copyright © 2023 Escalona, Larqué, Cortes, Vilchis, Granados-Delgado, Sánchez, Sánchez-Bringas and Lugo-Martínez https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Escalona, Rene
Larqué, Carlos
Cortes, Daniela
Vilchis, Ricardo
Granados-Delgado, Emiliano
Sánchez, Abigail
Sánchez-Bringas, Guadalupe
Lugo-Martínez, Haydée
High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
title High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
title_full High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
title_fullStr High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
title_full_unstemmed High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
title_short High-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
title_sort high-fat diet impairs glucose homeostasis by increased p16 beta-cell expression and alters glucose homeostasis of the progeny in a parental-sex dependent manner
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591070/
https://www.ncbi.nlm.nih.gov/pubmed/37876538
http://dx.doi.org/10.3389/fendo.2023.1246194
work_keys_str_mv AT escalonarene highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT larquecarlos highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT cortesdaniela highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT vilchisricardo highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT granadosdelgadoemiliano highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT sanchezabigail highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT sanchezbringasguadalupe highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner
AT lugomartinezhaydee highfatdietimpairsglucosehomeostasisbyincreasedp16betacellexpressionandaltersglucosehomeostasisoftheprogenyinaparentalsexdependentmanner