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Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles

Mapping functional connectivity between neurons is an essential step toward probing the neural computations mediating behavior. Accurately determining synaptic connectivity maps in populations of neurons is challenging in terms of yield, accuracy, and experimental time. Here, we developed a compress...

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Detalles Bibliográficos
Autores principales: Navarro, Phillip, Oweiss, Karim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591201/
https://www.ncbi.nlm.nih.gov/pubmed/37876895
http://dx.doi.org/10.1016/j.patter.2023.100845
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author Navarro, Phillip
Oweiss, Karim
author_facet Navarro, Phillip
Oweiss, Karim
author_sort Navarro, Phillip
collection PubMed
description Mapping functional connectivity between neurons is an essential step toward probing the neural computations mediating behavior. Accurately determining synaptic connectivity maps in populations of neurons is challenging in terms of yield, accuracy, and experimental time. Here, we developed a compressive sensing approach to reconstruct synaptic connectivity maps based on random two-photon cell-targeted optogenetic stimulation and membrane voltage readout of many putative postsynaptic neurons. Using a biophysical network model of interconnected populations of excitatory and inhibitory neurons, we characterized mapping recall and precision as a function of network observability, sparsity, number of neurons stimulated, off-target stimulation, synaptic reliability, propagation latency, and network topology. We found that mapping can be achieved with far fewer measurements than the standard pairwise sequential approach, with network sparsity and synaptic reliability serving as primary determinants of the performance. Our results suggest a rapid and efficient method to reconstruct functional connectivity of sparsely connected neuronal networks.
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spelling pubmed-105912012023-10-24 Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles Navarro, Phillip Oweiss, Karim Patterns (N Y) Article Mapping functional connectivity between neurons is an essential step toward probing the neural computations mediating behavior. Accurately determining synaptic connectivity maps in populations of neurons is challenging in terms of yield, accuracy, and experimental time. Here, we developed a compressive sensing approach to reconstruct synaptic connectivity maps based on random two-photon cell-targeted optogenetic stimulation and membrane voltage readout of many putative postsynaptic neurons. Using a biophysical network model of interconnected populations of excitatory and inhibitory neurons, we characterized mapping recall and precision as a function of network observability, sparsity, number of neurons stimulated, off-target stimulation, synaptic reliability, propagation latency, and network topology. We found that mapping can be achieved with far fewer measurements than the standard pairwise sequential approach, with network sparsity and synaptic reliability serving as primary determinants of the performance. Our results suggest a rapid and efficient method to reconstruct functional connectivity of sparsely connected neuronal networks. Elsevier 2023-09-22 /pmc/articles/PMC10591201/ /pubmed/37876895 http://dx.doi.org/10.1016/j.patter.2023.100845 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Navarro, Phillip
Oweiss, Karim
Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
title Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
title_full Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
title_fullStr Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
title_full_unstemmed Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
title_short Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
title_sort compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591201/
https://www.ncbi.nlm.nih.gov/pubmed/37876895
http://dx.doi.org/10.1016/j.patter.2023.100845
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