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A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions

Preventing L. monocytogenes infection is crucial for food safety, considering its widespread presence in the environment and its association with contaminated RTE foods. The pathogen's ability to persist under adverse conditions, for example, in food processing facilities, is linked to virulenc...

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Autores principales: D'Onofrio, Federica, Schirone, Maria, Krasteva, Ivanka, Tittarelli, Manuela, Iannetti, Luigi, Pomilio, Francesco, Torresi, Marina, Paparella, Antonello, D'Alterio, Nicola, Luciani, Mirella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591339/
https://www.ncbi.nlm.nih.gov/pubmed/37876777
http://dx.doi.org/10.3389/fmicb.2023.1271787
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author D'Onofrio, Federica
Schirone, Maria
Krasteva, Ivanka
Tittarelli, Manuela
Iannetti, Luigi
Pomilio, Francesco
Torresi, Marina
Paparella, Antonello
D'Alterio, Nicola
Luciani, Mirella
author_facet D'Onofrio, Federica
Schirone, Maria
Krasteva, Ivanka
Tittarelli, Manuela
Iannetti, Luigi
Pomilio, Francesco
Torresi, Marina
Paparella, Antonello
D'Alterio, Nicola
Luciani, Mirella
author_sort D'Onofrio, Federica
collection PubMed
description Preventing L. monocytogenes infection is crucial for food safety, considering its widespread presence in the environment and its association with contaminated RTE foods. The pathogen's ability to persist under adverse conditions, for example, in food processing facilities, is linked to virulence and resistance mechanisms, including biofilm formation. In this study, the protein expression patterns of two L. monocytogenes 1/2a strains, grown under environmental stressors (mild acidic pH, thermal abuse, and high concentration of NaCl), were investigated. Protein identification and prediction were performed by nLC-ESI-MS/MS and nine different bioinformatic software programs, respectively. Gene enrichment analysis was carried out by STRING v11.05. A total of 1,215 proteins were identified, of which 335 were non-cytosolic proteins and 265 were immunogenic proteins. Proteomic analysis revealed differences in protein expression between L. monocytogenes strains in stressful conditions. The two strains exhibited unique protein expression profiles linked to stress response, virulence, and pathogenesis. Studying the proteomic profiles of such microorganisms provides information about adaptation and potential treatments, highlighting their genetic diversity and demonstrating the utility of bioinformatics and proteomics for a broader analysis of pathogens.
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spelling pubmed-105913392023-10-24 A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions D'Onofrio, Federica Schirone, Maria Krasteva, Ivanka Tittarelli, Manuela Iannetti, Luigi Pomilio, Francesco Torresi, Marina Paparella, Antonello D'Alterio, Nicola Luciani, Mirella Front Microbiol Microbiology Preventing L. monocytogenes infection is crucial for food safety, considering its widespread presence in the environment and its association with contaminated RTE foods. The pathogen's ability to persist under adverse conditions, for example, in food processing facilities, is linked to virulence and resistance mechanisms, including biofilm formation. In this study, the protein expression patterns of two L. monocytogenes 1/2a strains, grown under environmental stressors (mild acidic pH, thermal abuse, and high concentration of NaCl), were investigated. Protein identification and prediction were performed by nLC-ESI-MS/MS and nine different bioinformatic software programs, respectively. Gene enrichment analysis was carried out by STRING v11.05. A total of 1,215 proteins were identified, of which 335 were non-cytosolic proteins and 265 were immunogenic proteins. Proteomic analysis revealed differences in protein expression between L. monocytogenes strains in stressful conditions. The two strains exhibited unique protein expression profiles linked to stress response, virulence, and pathogenesis. Studying the proteomic profiles of such microorganisms provides information about adaptation and potential treatments, highlighting their genetic diversity and demonstrating the utility of bioinformatics and proteomics for a broader analysis of pathogens. Frontiers Media S.A. 2023-10-09 /pmc/articles/PMC10591339/ /pubmed/37876777 http://dx.doi.org/10.3389/fmicb.2023.1271787 Text en Copyright © 2023 D'Onofrio, Schirone, Krasteva, Tittarelli, Iannetti, Pomilio, Torresi, Paparella, D'Alterio and Luciani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
D'Onofrio, Federica
Schirone, Maria
Krasteva, Ivanka
Tittarelli, Manuela
Iannetti, Luigi
Pomilio, Francesco
Torresi, Marina
Paparella, Antonello
D'Alterio, Nicola
Luciani, Mirella
A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
title A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
title_full A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
title_fullStr A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
title_full_unstemmed A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
title_short A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
title_sort comprehensive investigation of protein expression profiles in l. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591339/
https://www.ncbi.nlm.nih.gov/pubmed/37876777
http://dx.doi.org/10.3389/fmicb.2023.1271787
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