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A comparative analysis of microglial inducible Cre lines
Cre/loxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. Microglial biology has particularly benefited because microglia historically have been difficult to transduce with virus or electroporation methods for gene...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591718/ https://www.ncbi.nlm.nih.gov/pubmed/37635351 http://dx.doi.org/10.1016/j.celrep.2023.113031 |
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author | Faust, Travis E. Feinberg, Philip A. O’Connor, Ciara Kawaguchi, Riki Chan, Andrew Strasburger, Hayley Frosch, Maximilian Boyle, Margaret A. Masuda, Takahiro Amann, Lukas Knobeloch, Klaus-Peter Prinz, Marco Schaefer, Anne Schafer, Dorothy P. |
author_facet | Faust, Travis E. Feinberg, Philip A. O’Connor, Ciara Kawaguchi, Riki Chan, Andrew Strasburger, Hayley Frosch, Maximilian Boyle, Margaret A. Masuda, Takahiro Amann, Lukas Knobeloch, Klaus-Peter Prinz, Marco Schaefer, Anne Schafer, Dorothy P. |
author_sort | Faust, Travis E. |
collection | PubMed |
description | Cre/loxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. Microglial biology has particularly benefited because microglia historically have been difficult to transduce with virus or electroporation methods for gene delivery. Here, we investigate five of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency, cell-type specificity, and spontaneous recombination, depending on the Cre line and inter-loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency, particularly in microglia. There is increasing evidence that microglia are key regulators of neural circuits and major drivers of a broad range of neurological diseases. Reliable manipulation of their function in vivo is of utmost importance. Identifying caveats and benefits of all tools and implementing the most rigorous protocols are crucial to the growth of the field and the development of microglia-based therapeutics. |
format | Online Article Text |
id | pubmed-10591718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-105917182023-10-23 A comparative analysis of microglial inducible Cre lines Faust, Travis E. Feinberg, Philip A. O’Connor, Ciara Kawaguchi, Riki Chan, Andrew Strasburger, Hayley Frosch, Maximilian Boyle, Margaret A. Masuda, Takahiro Amann, Lukas Knobeloch, Klaus-Peter Prinz, Marco Schaefer, Anne Schafer, Dorothy P. Cell Rep Article Cre/loxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. Microglial biology has particularly benefited because microglia historically have been difficult to transduce with virus or electroporation methods for gene delivery. Here, we investigate five of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency, cell-type specificity, and spontaneous recombination, depending on the Cre line and inter-loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency, particularly in microglia. There is increasing evidence that microglia are key regulators of neural circuits and major drivers of a broad range of neurological diseases. Reliable manipulation of their function in vivo is of utmost importance. Identifying caveats and benefits of all tools and implementing the most rigorous protocols are crucial to the growth of the field and the development of microglia-based therapeutics. 2023-09-26 2023-08-26 /pmc/articles/PMC10591718/ /pubmed/37635351 http://dx.doi.org/10.1016/j.celrep.2023.113031 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Faust, Travis E. Feinberg, Philip A. O’Connor, Ciara Kawaguchi, Riki Chan, Andrew Strasburger, Hayley Frosch, Maximilian Boyle, Margaret A. Masuda, Takahiro Amann, Lukas Knobeloch, Klaus-Peter Prinz, Marco Schaefer, Anne Schafer, Dorothy P. A comparative analysis of microglial inducible Cre lines |
title | A comparative analysis of microglial inducible Cre lines |
title_full | A comparative analysis of microglial inducible Cre lines |
title_fullStr | A comparative analysis of microglial inducible Cre lines |
title_full_unstemmed | A comparative analysis of microglial inducible Cre lines |
title_short | A comparative analysis of microglial inducible Cre lines |
title_sort | comparative analysis of microglial inducible cre lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591718/ https://www.ncbi.nlm.nih.gov/pubmed/37635351 http://dx.doi.org/10.1016/j.celrep.2023.113031 |
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