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LIF signaling regulates outer radial glial to interneuron fate during human cortical development
Radial glial (RG) development is essential for cerebral cortex growth and organization. In humans, the outer radial glia (oRG) subtype is expanded and gives rise to diverse neurons and glia. However, the mechanisms regulating oRG differentiation are unclear. oRG cells express leukemia-inhibitory fac...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591955/ https://www.ncbi.nlm.nih.gov/pubmed/37673072 http://dx.doi.org/10.1016/j.stem.2023.08.009 |
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author | Andrews, Madeline G. Siebert, Clara Wang, Li White, Matthew L. Ross, Jayden Morales, Raul Donnay, Megan Bamfonga, Gradi Mukhtar, Tanzila McKinney, Arpana Arjun Gemenes, Kaila Wang, Shaohui Bi, Qiuli Crouch, Elizabeth E. Parikshak, Neelroop Panagiotakos, Georgia Huang, Eric Bhaduri, Aparna Kriegstein, Arnold R. |
author_facet | Andrews, Madeline G. Siebert, Clara Wang, Li White, Matthew L. Ross, Jayden Morales, Raul Donnay, Megan Bamfonga, Gradi Mukhtar, Tanzila McKinney, Arpana Arjun Gemenes, Kaila Wang, Shaohui Bi, Qiuli Crouch, Elizabeth E. Parikshak, Neelroop Panagiotakos, Georgia Huang, Eric Bhaduri, Aparna Kriegstein, Arnold R. |
author_sort | Andrews, Madeline G. |
collection | PubMed |
description | Radial glial (RG) development is essential for cerebral cortex growth and organization. In humans, the outer radial glia (oRG) subtype is expanded and gives rise to diverse neurons and glia. However, the mechanisms regulating oRG differentiation are unclear. oRG cells express leukemia-inhibitory factor (LIF) receptors during neurogenesis, and consistent with a role in stem cell self-renewal, LIF perturbation impacts oRG proliferation in cortical tissue and organoids. Surprisingly, LIF treatment also increases the production of inhibitory interneurons (INs) in cortical cultures. Comparative transcriptomic analysis identifies that the enhanced IN population resembles INs produced in the caudal ganglionic eminence. To evaluate whether INs could arise from oRGs, we isolated primary oRG cells and cultured them with LIF. We observed the production of INs from oRG cells and an increase in IN abundance following LIF treatment. Our observations suggest that LIF signaling regulates the capacity of oRG cells to generate INs. |
format | Online Article Text |
id | pubmed-10591955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-105919552023-10-23 LIF signaling regulates outer radial glial to interneuron fate during human cortical development Andrews, Madeline G. Siebert, Clara Wang, Li White, Matthew L. Ross, Jayden Morales, Raul Donnay, Megan Bamfonga, Gradi Mukhtar, Tanzila McKinney, Arpana Arjun Gemenes, Kaila Wang, Shaohui Bi, Qiuli Crouch, Elizabeth E. Parikshak, Neelroop Panagiotakos, Georgia Huang, Eric Bhaduri, Aparna Kriegstein, Arnold R. Cell Stem Cell Article Radial glial (RG) development is essential for cerebral cortex growth and organization. In humans, the outer radial glia (oRG) subtype is expanded and gives rise to diverse neurons and glia. However, the mechanisms regulating oRG differentiation are unclear. oRG cells express leukemia-inhibitory factor (LIF) receptors during neurogenesis, and consistent with a role in stem cell self-renewal, LIF perturbation impacts oRG proliferation in cortical tissue and organoids. Surprisingly, LIF treatment also increases the production of inhibitory interneurons (INs) in cortical cultures. Comparative transcriptomic analysis identifies that the enhanced IN population resembles INs produced in the caudal ganglionic eminence. To evaluate whether INs could arise from oRGs, we isolated primary oRG cells and cultured them with LIF. We observed the production of INs from oRG cells and an increase in IN abundance following LIF treatment. Our observations suggest that LIF signaling regulates the capacity of oRG cells to generate INs. 2023-10-05 2023-09-05 /pmc/articles/PMC10591955/ /pubmed/37673072 http://dx.doi.org/10.1016/j.stem.2023.08.009 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ). |
spellingShingle | Article Andrews, Madeline G. Siebert, Clara Wang, Li White, Matthew L. Ross, Jayden Morales, Raul Donnay, Megan Bamfonga, Gradi Mukhtar, Tanzila McKinney, Arpana Arjun Gemenes, Kaila Wang, Shaohui Bi, Qiuli Crouch, Elizabeth E. Parikshak, Neelroop Panagiotakos, Georgia Huang, Eric Bhaduri, Aparna Kriegstein, Arnold R. LIF signaling regulates outer radial glial to interneuron fate during human cortical development |
title | LIF signaling regulates outer radial glial to interneuron fate during human cortical development |
title_full | LIF signaling regulates outer radial glial to interneuron fate during human cortical development |
title_fullStr | LIF signaling regulates outer radial glial to interneuron fate during human cortical development |
title_full_unstemmed | LIF signaling regulates outer radial glial to interneuron fate during human cortical development |
title_short | LIF signaling regulates outer radial glial to interneuron fate during human cortical development |
title_sort | lif signaling regulates outer radial glial to interneuron fate during human cortical development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591955/ https://www.ncbi.nlm.nih.gov/pubmed/37673072 http://dx.doi.org/10.1016/j.stem.2023.08.009 |
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