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Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly

The superior colliculus (SC) is a sensorimotor structure in the midbrain that integrates input from multiple sensory modalities to initiate motor commands. It undergoes well-characterized steps of circuit assembly during development, rendering the mouse SC a popular model to study establishment of n...

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Autores principales: Choi, James S., Ayupe, Ana C., Beckedorff, Felipe, Catanuto, Paola, McCartan, Robyn, Levay, Konstantin, Park, Kevin K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592058/
https://www.ncbi.nlm.nih.gov/pubmed/37624694
http://dx.doi.org/10.1016/j.celrep.2023.113037
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author Choi, James S.
Ayupe, Ana C.
Beckedorff, Felipe
Catanuto, Paola
McCartan, Robyn
Levay, Konstantin
Park, Kevin K.
author_facet Choi, James S.
Ayupe, Ana C.
Beckedorff, Felipe
Catanuto, Paola
McCartan, Robyn
Levay, Konstantin
Park, Kevin K.
author_sort Choi, James S.
collection PubMed
description The superior colliculus (SC) is a sensorimotor structure in the midbrain that integrates input from multiple sensory modalities to initiate motor commands. It undergoes well-characterized steps of circuit assembly during development, rendering the mouse SC a popular model to study establishment of neural connectivity. Here we perform single-nucleus RNA-sequencing analysis of the mouse SC isolated at various developmental time points. Our study provides a transcriptomic landscape of the cell types that comprise the SC across murine development with particular emphasis on neuronal heterogeneity. We report a repertoire of genes differentially expressed across the different postnatal ages, many of which are known to regulate axon guidance and synapse formation. Using these data, we find that Pax7 expression is restricted to a subset of GABAergic neurons. Our data provide a valuable resource for interrogating the mechanisms of circuit development and identifying markers for manipulating specific SC neuronal populations and circuits.
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spelling pubmed-105920582023-10-23 Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly Choi, James S. Ayupe, Ana C. Beckedorff, Felipe Catanuto, Paola McCartan, Robyn Levay, Konstantin Park, Kevin K. Cell Rep Article The superior colliculus (SC) is a sensorimotor structure in the midbrain that integrates input from multiple sensory modalities to initiate motor commands. It undergoes well-characterized steps of circuit assembly during development, rendering the mouse SC a popular model to study establishment of neural connectivity. Here we perform single-nucleus RNA-sequencing analysis of the mouse SC isolated at various developmental time points. Our study provides a transcriptomic landscape of the cell types that comprise the SC across murine development with particular emphasis on neuronal heterogeneity. We report a repertoire of genes differentially expressed across the different postnatal ages, many of which are known to regulate axon guidance and synapse formation. Using these data, we find that Pax7 expression is restricted to a subset of GABAergic neurons. Our data provide a valuable resource for interrogating the mechanisms of circuit development and identifying markers for manipulating specific SC neuronal populations and circuits. 2023-09-26 2023-08-24 /pmc/articles/PMC10592058/ /pubmed/37624694 http://dx.doi.org/10.1016/j.celrep.2023.113037 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Choi, James S.
Ayupe, Ana C.
Beckedorff, Felipe
Catanuto, Paola
McCartan, Robyn
Levay, Konstantin
Park, Kevin K.
Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
title Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
title_full Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
title_fullStr Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
title_full_unstemmed Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
title_short Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
title_sort single-nucleus rna sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592058/
https://www.ncbi.nlm.nih.gov/pubmed/37624694
http://dx.doi.org/10.1016/j.celrep.2023.113037
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