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Scanning mutagenesis of the voltage-gated sodium channel Na(V)1.2 using base editing

It is challenging to apply traditional mutational scanning to voltage-gated sodium channels (Na(V)s) and functionally annotate the large number of coding variants in these genes. Using a cytosine base editor and a pooled viability assay, we screen a library of 368 guide RNAs (gRNAs) tiling Na(V)1.2...

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Detalles Bibliográficos
Autores principales:	Pablo, Juan Lorenzo B., Cornett, Savannah L., Wang, Lei A., Jo, Sooyeon, Brünger, Tobias, Budnik, Nikita, Hegde, Mudra, DeKeyser, Jean-Marc, Thompson, Christopher H., Doench, John G., Lal, Dennis, George, Alfred L., Pan, Jen Q.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592450/ https://www.ncbi.nlm.nih.gov/pubmed/37267104 http://dx.doi.org/10.1016/j.celrep.2023.112563

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