Square beams for optimal tiling in TEM

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Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square elect...

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Autores principales: Chua, Eugene YD, Alink, Lambertus M, Kopylov, Mykhailo, Johnston, Jake, Eisenstein, Fabian, de Marco, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592621/
https://www.ncbi.nlm.nih.gov/pubmed/37873376
http://dx.doi.org/10.1101/2023.07.29.551095
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author Chua, Eugene YD
Alink, Lambertus M
Kopylov, Mykhailo
Johnston, Jake
Eisenstein, Fabian
de Marco, Alex
author_facet Chua, Eugene YD
Alink, Lambertus M
Kopylov, Mykhailo
Johnston, Jake
Eisenstein, Fabian
de Marco, Alex
author_sort Chua, Eugene YD
collection PubMed
description Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application, showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups.
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spelling pubmed-105926212023-10-24 Square beams for optimal tiling in TEM Chua, Eugene YD Alink, Lambertus M Kopylov, Mykhailo Johnston, Jake Eisenstein, Fabian de Marco, Alex bioRxiv Article Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application, showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups. Cold Spring Harbor Laboratory 2023-10-09 /pmc/articles/PMC10592621/ /pubmed/37873376 http://dx.doi.org/10.1101/2023.07.29.551095 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Chua, Eugene YD
Alink, Lambertus M
Kopylov, Mykhailo
Johnston, Jake
Eisenstein, Fabian
de Marco, Alex
Square beams for optimal tiling in TEM
title Square beams for optimal tiling in TEM
title_full Square beams for optimal tiling in TEM
title_fullStr Square beams for optimal tiling in TEM
title_full_unstemmed Square beams for optimal tiling in TEM
title_short Square beams for optimal tiling in TEM
title_sort square beams for optimal tiling in tem
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592621/
https://www.ncbi.nlm.nih.gov/pubmed/37873376
http://dx.doi.org/10.1101/2023.07.29.551095
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