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TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT
Male germ cell development is dependent on the orchestrated regulation of gene networks. TATA-box binding protein associated factors (TAFs) facilitate interactions of TATA-binding protein with the TATA element, which is known to coordinate gene transcription during organogenesis. TAF7 like (Taf7l) i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592675/ https://www.ncbi.nlm.nih.gov/pubmed/37873461 http://dx.doi.org/10.1101/2023.10.08.561408 |
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author | Moreno-Irusta, Ayelen Dominguez, Esteban M. Iqbal, Khursheed Zhang, Xiaoyu Wang, Ning Soares, Michael J. |
author_facet | Moreno-Irusta, Ayelen Dominguez, Esteban M. Iqbal, Khursheed Zhang, Xiaoyu Wang, Ning Soares, Michael J. |
author_sort | Moreno-Irusta, Ayelen |
collection | PubMed |
description | Male germ cell development is dependent on the orchestrated regulation of gene networks. TATA-box binding protein associated factors (TAFs) facilitate interactions of TATA-binding protein with the TATA element, which is known to coordinate gene transcription during organogenesis. TAF7 like (Taf7l) is situated on the X chromosome and has been implicated in testis development. We examined the biology of TAF7L in testis development using the rat. Taf7l was prominently expressed in preleptotene to leptotene spermatocytes. To study the impact of TAF7L on the testis we generated a global loss-of-function rat model using CRISPR/Cas9 genome editing. Exon 3 of the Taf7l gene was targeted. A founder was generated possessing a 110 bp deletion within the Taf7l locus, which resulted in a frameshift and the premature appearance of a stop codon. The mutation was effectively transmitted through the germline. Deficits in TAF7L did not adversely affect pregnancy or postnatal survival. However, the Taf7l disruption resulted in male infertility due to compromised testis development and failed sperm production. Mutant germ cells suffer meiotic arrest at the zygotene stage, with defects in sex body formation and meiotic sex chromosome inactivation. This testis phenotype was more pronounced than previously described for the subfertile Taf7l null mouse. We conclude that TAF7L is essential for male germ cell development in the rat. |
format | Online Article Text |
id | pubmed-10592675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-105926752023-10-24 TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT Moreno-Irusta, Ayelen Dominguez, Esteban M. Iqbal, Khursheed Zhang, Xiaoyu Wang, Ning Soares, Michael J. bioRxiv Article Male germ cell development is dependent on the orchestrated regulation of gene networks. TATA-box binding protein associated factors (TAFs) facilitate interactions of TATA-binding protein with the TATA element, which is known to coordinate gene transcription during organogenesis. TAF7 like (Taf7l) is situated on the X chromosome and has been implicated in testis development. We examined the biology of TAF7L in testis development using the rat. Taf7l was prominently expressed in preleptotene to leptotene spermatocytes. To study the impact of TAF7L on the testis we generated a global loss-of-function rat model using CRISPR/Cas9 genome editing. Exon 3 of the Taf7l gene was targeted. A founder was generated possessing a 110 bp deletion within the Taf7l locus, which resulted in a frameshift and the premature appearance of a stop codon. The mutation was effectively transmitted through the germline. Deficits in TAF7L did not adversely affect pregnancy or postnatal survival. However, the Taf7l disruption resulted in male infertility due to compromised testis development and failed sperm production. Mutant germ cells suffer meiotic arrest at the zygotene stage, with defects in sex body formation and meiotic sex chromosome inactivation. This testis phenotype was more pronounced than previously described for the subfertile Taf7l null mouse. We conclude that TAF7L is essential for male germ cell development in the rat. Cold Spring Harbor Laboratory 2023-10-10 /pmc/articles/PMC10592675/ /pubmed/37873461 http://dx.doi.org/10.1101/2023.10.08.561408 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Moreno-Irusta, Ayelen Dominguez, Esteban M. Iqbal, Khursheed Zhang, Xiaoyu Wang, Ning Soares, Michael J. TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT |
title | TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT |
title_full | TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT |
title_fullStr | TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT |
title_full_unstemmed | TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT |
title_short | TAF7L REGULATES EARLY STAGES OF MALE GERM CELL DEVELOPMENT |
title_sort | taf7l regulates early stages of male germ cell development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592675/ https://www.ncbi.nlm.nih.gov/pubmed/37873461 http://dx.doi.org/10.1101/2023.10.08.561408 |
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