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Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina
To clarify our understanding of glial phagocytosis in retinal development, we used real time imaging of larval zebrafish to provide cell-type specific resolution of this process. We show that radial Müller glia frequently participate in microglial phagocytosis while also completing a subset of phago...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592698/ https://www.ncbi.nlm.nih.gov/pubmed/37873206 http://dx.doi.org/10.1101/2023.10.06.561302 |
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author | Morales, Michael Findley, Anna P Mitchell, Diana M. |
author_facet | Morales, Michael Findley, Anna P Mitchell, Diana M. |
author_sort | Morales, Michael |
collection | PubMed |
description | To clarify our understanding of glial phagocytosis in retinal development, we used real time imaging of larval zebrafish to provide cell-type specific resolution of this process. We show that radial Müller glia frequently participate in microglial phagocytosis while also completing a subset of phagocytic events. Müller glia (MG) actively engage with dying cells through initial target cell contact and phagocytic cup formation after which an exchange of the dying cell from MG to microglia often takes place. Additionally, we find evidence that Müller glia cellular material, possibly from the initial Müller cell’s phagocytic cup, is internalized into microglial compartments. Previously undescribed Müller cell behaviors were seen, including cargo splitting, wrestling for targets, lateral passing of cargo to neighbors, and engulfment of what is possibly synaptic puncta. Collectively, our work provides new insight into glial functions and intercellular interactions, which will allow future work to understand these behaviors on a molecular level. |
format | Online Article Text |
id | pubmed-10592698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-105926982023-10-24 Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina Morales, Michael Findley, Anna P Mitchell, Diana M. bioRxiv Article To clarify our understanding of glial phagocytosis in retinal development, we used real time imaging of larval zebrafish to provide cell-type specific resolution of this process. We show that radial Müller glia frequently participate in microglial phagocytosis while also completing a subset of phagocytic events. Müller glia (MG) actively engage with dying cells through initial target cell contact and phagocytic cup formation after which an exchange of the dying cell from MG to microglia often takes place. Additionally, we find evidence that Müller glia cellular material, possibly from the initial Müller cell’s phagocytic cup, is internalized into microglial compartments. Previously undescribed Müller cell behaviors were seen, including cargo splitting, wrestling for targets, lateral passing of cargo to neighbors, and engulfment of what is possibly synaptic puncta. Collectively, our work provides new insight into glial functions and intercellular interactions, which will allow future work to understand these behaviors on a molecular level. Cold Spring Harbor Laboratory 2023-10-09 /pmc/articles/PMC10592698/ /pubmed/37873206 http://dx.doi.org/10.1101/2023.10.06.561302 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Morales, Michael Findley, Anna P Mitchell, Diana M. Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina |
title | Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina |
title_full | Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina |
title_fullStr | Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina |
title_full_unstemmed | Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina |
title_short | Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina |
title_sort | intercellular contact and cargo transfer between müller glia and to microglia precede apoptotic cell clearance in the developing retina |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592698/ https://www.ncbi.nlm.nih.gov/pubmed/37873206 http://dx.doi.org/10.1101/2023.10.06.561302 |
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