Arrayed in vivo barcoding for multiplexed sequence verification of plasmid DNA and demultiplexing of pooled libraries

Sequence verification of plasmid DNA is critical for many cloning and molecular biology workflows. To leverage high-throughput sequencing, several methods have been developed that add a unique DNA barcode to individual samples prior to pooling and sequencing. However, these methods require an indivi...

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Detalles Bibliográficos
Autores principales: Li, Weiyi, Miller, Darach, Liu, Xianan, Tosi, Lorenzo, Chkaiban, Lamia, Mei, Han, Hung, Po-Hsiang, Parekkadan, Biju, Sherlock, Gavin, Levy, Sasha F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592806/
https://www.ncbi.nlm.nih.gov/pubmed/37873145
http://dx.doi.org/10.1101/2023.10.13.562064

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