Introduction of Dicistrovirus IRESs into UAS/SV40-polyA constructs results in premature polyadenylation and strong overexpression of the upstream ORF in Drosophila animals

To evaluate the properties of insect virus internal ribosomal entry sites (IRESs) for protein expression in Drosophila, we have introduced Cricket Paralysis virus (CrPV) and Drosophila C virus (DCV) IRESs into UAS/SV40-polyA vector. We found that introduction of IRESs induce premature polyadenylatio...

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Detalles Bibliográficos
Autores principales: Lidsky, Peter V., Dmitriev, Sergey E., Andino, Raul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592961/
https://www.ncbi.nlm.nih.gov/pubmed/37873388
http://dx.doi.org/10.1101/2023.10.04.560905

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592961/
https://www.ncbi.nlm.nih.gov/pubmed/37873388
http://dx.doi.org/10.1101/2023.10.04.560905

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