Cargando…
Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates
The obligate biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals, which results in large crop losses. Control of B. graminis in barley is mainly achieved by fungicide treatment and by breeding resistant varieties. Vampyrellid amoebae, just like mycophagous proti...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593611/ https://www.ncbi.nlm.nih.gov/pubmed/37872440 http://dx.doi.org/10.1007/s00284-023-03497-5 |
_version_ | 1785124479580504064 |
---|---|
author | Sacharow, Julia Salehi-Mobarakeh, Elnaz Ratering, Stefan Imani, Jafargholi Österreicher Cunha-Dupont, Alessandra Schnell, Sylvia |
author_facet | Sacharow, Julia Salehi-Mobarakeh, Elnaz Ratering, Stefan Imani, Jafargholi Österreicher Cunha-Dupont, Alessandra Schnell, Sylvia |
author_sort | Sacharow, Julia |
collection | PubMed |
description | The obligate biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals, which results in large crop losses. Control of B. graminis in barley is mainly achieved by fungicide treatment and by breeding resistant varieties. Vampyrellid amoebae, just like mycophagous protists, are able to consume a variety of fungi. To reveal the impact of some selected fungus-consuming protists on Blumeria graminis f. sp. hordei (Bgh), and to evaluate the possibility of using these protists as biological agents in the future, their feeding behaviour on B. graminis spores on barley leaves was investigated. An experiment was carried out with five different protist isolates (Leptophrys vorax, Platyreta germanica, Theratromyxa weberi U 11, Theratromyxa weberi G7.2 and Acanthamoeba castellanii) and four matched controls, including the food sources of the cultures and the medium. Ten-day-old leaves of barley (Hordeum vulgare cv. Golden Promise) were first inoculated with Blumeria graminis (f. sp. hordei race A6) spores, then treated with protists and fungal colonies on the leaf surfaces were counted under the microscope after 5 days. The isolates L. vorax, P. germanica, and T. weberi U11 did not show a significant reduction in the number of powdery mildew colonies whereas the isolates T. weberi G7.2 and A. castellanii significantly reduced the number of powdery mildew colonies on the leaf surfaces compared to their respective controls. This indicates that these two isolates are capable of reducing B. graminis colonies on barley leaves and are suitable candidates for further investigation for possible use as biological agents. Nevertheless, the susceptibility to dryness and the cell division rate should be considered during the optimisation of the next steps like application procedure and whole plant treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-023-03497-5. |
format | Online Article Text |
id | pubmed-10593611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-105936112023-10-25 Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates Sacharow, Julia Salehi-Mobarakeh, Elnaz Ratering, Stefan Imani, Jafargholi Österreicher Cunha-Dupont, Alessandra Schnell, Sylvia Curr Microbiol Article The obligate biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals, which results in large crop losses. Control of B. graminis in barley is mainly achieved by fungicide treatment and by breeding resistant varieties. Vampyrellid amoebae, just like mycophagous protists, are able to consume a variety of fungi. To reveal the impact of some selected fungus-consuming protists on Blumeria graminis f. sp. hordei (Bgh), and to evaluate the possibility of using these protists as biological agents in the future, their feeding behaviour on B. graminis spores on barley leaves was investigated. An experiment was carried out with five different protist isolates (Leptophrys vorax, Platyreta germanica, Theratromyxa weberi U 11, Theratromyxa weberi G7.2 and Acanthamoeba castellanii) and four matched controls, including the food sources of the cultures and the medium. Ten-day-old leaves of barley (Hordeum vulgare cv. Golden Promise) were first inoculated with Blumeria graminis (f. sp. hordei race A6) spores, then treated with protists and fungal colonies on the leaf surfaces were counted under the microscope after 5 days. The isolates L. vorax, P. germanica, and T. weberi U11 did not show a significant reduction in the number of powdery mildew colonies whereas the isolates T. weberi G7.2 and A. castellanii significantly reduced the number of powdery mildew colonies on the leaf surfaces compared to their respective controls. This indicates that these two isolates are capable of reducing B. graminis colonies on barley leaves and are suitable candidates for further investigation for possible use as biological agents. Nevertheless, the susceptibility to dryness and the cell division rate should be considered during the optimisation of the next steps like application procedure and whole plant treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-023-03497-5. Springer US 2023-10-23 2023 /pmc/articles/PMC10593611/ /pubmed/37872440 http://dx.doi.org/10.1007/s00284-023-03497-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sacharow, Julia Salehi-Mobarakeh, Elnaz Ratering, Stefan Imani, Jafargholi Österreicher Cunha-Dupont, Alessandra Schnell, Sylvia Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates |
title | Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates |
title_full | Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates |
title_fullStr | Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates |
title_full_unstemmed | Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates |
title_short | Control of Blumeria graminis f. sp. hordei on Barley Leaves by Treatment with Fungi-Consuming Protist Isolates |
title_sort | control of blumeria graminis f. sp. hordei on barley leaves by treatment with fungi-consuming protist isolates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593611/ https://www.ncbi.nlm.nih.gov/pubmed/37872440 http://dx.doi.org/10.1007/s00284-023-03497-5 |
work_keys_str_mv | AT sacharowjulia controlofblumeriagraminisfsphordeionbarleyleavesbytreatmentwithfungiconsumingprotistisolates AT salehimobarakehelnaz controlofblumeriagraminisfsphordeionbarleyleavesbytreatmentwithfungiconsumingprotistisolates AT rateringstefan controlofblumeriagraminisfsphordeionbarleyleavesbytreatmentwithfungiconsumingprotistisolates AT imanijafargholi controlofblumeriagraminisfsphordeionbarleyleavesbytreatmentwithfungiconsumingprotistisolates AT osterreichercunhadupontalessandra controlofblumeriagraminisfsphordeionbarleyleavesbytreatmentwithfungiconsumingprotistisolates AT schnellsylvia controlofblumeriagraminisfsphordeionbarleyleavesbytreatmentwithfungiconsumingprotistisolates |