Cargando…
MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism
Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. T...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593791/ https://www.ncbi.nlm.nih.gov/pubmed/37872134 http://dx.doi.org/10.1038/s41467-023-42511-z |
_version_ | 1785124507427536896 |
---|---|
author | Dluzewska, Julia Dziegielewski, Wojciech Szymanska-Lejman, Maja Gazecka, Monika Henderson, Ian R. Higgins, James D. Ziolkowski, Piotr A. |
author_facet | Dluzewska, Julia Dziegielewski, Wojciech Szymanska-Lejman, Maja Gazecka, Monika Henderson, Ian R. Higgins, James D. Ziolkowski, Piotr A. |
author_sort | Dluzewska, Julia |
collection | PubMed |
description | Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism. |
format | Online Article Text |
id | pubmed-10593791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105937912023-10-25 MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism Dluzewska, Julia Dziegielewski, Wojciech Szymanska-Lejman, Maja Gazecka, Monika Henderson, Ian R. Higgins, James D. Ziolkowski, Piotr A. Nat Commun Article Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism. Nature Publishing Group UK 2023-10-23 /pmc/articles/PMC10593791/ /pubmed/37872134 http://dx.doi.org/10.1038/s41467-023-42511-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Dluzewska, Julia Dziegielewski, Wojciech Szymanska-Lejman, Maja Gazecka, Monika Henderson, Ian R. Higgins, James D. Ziolkowski, Piotr A. MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
title | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
title_full | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
title_fullStr | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
title_full_unstemmed | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
title_short | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
title_sort | msh2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593791/ https://www.ncbi.nlm.nih.gov/pubmed/37872134 http://dx.doi.org/10.1038/s41467-023-42511-z |
work_keys_str_mv | AT dluzewskajulia msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism AT dziegielewskiwojciech msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism AT szymanskalejmanmaja msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism AT gazeckamonika msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism AT hendersonianr msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism AT higginsjamesd msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism AT ziolkowskipiotra msh2stimulatesinterferingandinhibitsnoninterferingcrossoversinresponsetogeneticpolymorphism |