Cargando…

Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice

The Haemophilus influenzae type B (Hib) conjugate vaccine is the most effective way to prevent Hib infection in infants and young children, and it is designed to induce the production of antibodies against polyribosylribitol phosphate (PRP) to protect babies from infection. However, the mechanism of...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Yao, Zhao, Yuxiu, Liang, Hongyang, Wang, Xue, Lan, Haoyue, Tian, Dongyang, Li, Yan, Wang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594484/
https://www.ncbi.nlm.nih.gov/pubmed/37873783
http://dx.doi.org/10.3390/diseases11040139
_version_ 1785124660871954432
author He, Yao
Zhao, Yuxiu
Liang, Hongyang
Wang, Xue
Lan, Haoyue
Tian, Dongyang
Li, Yan
Wang, Hui
author_facet He, Yao
Zhao, Yuxiu
Liang, Hongyang
Wang, Xue
Lan, Haoyue
Tian, Dongyang
Li, Yan
Wang, Hui
author_sort He, Yao
collection PubMed
description The Haemophilus influenzae type B (Hib) conjugate vaccine is the most effective way to prevent Hib infection in infants and young children, and it is designed to induce the production of antibodies against polyribosylribitol phosphate (PRP) to protect babies from infection. However, the mechanism of immunity induced by the Hib vaccine is not fully understood. Recently, with the development of the combination diphtheria and tetanus toxoids and acellular pertussis vaccines (DTaP), increasing numbers of manufacturers have begun to develop DTaP-based combination vaccines, like the combination vaccine diphtheria and tetanus toxoids and acellular pertussis and Hib conjugate vaccine (DTaP-Hib), which contains adjuvants. However, the Hib vaccine does not contain adjuvants. It was theorized that the Hib antigen has poor compatibility with aluminum adjuvants for unclear reasons. Therefore, understanding the mechanism of the Hib-vaccine-induced immune response and the influence of adjuvants on the Hib vaccine is of great significance. In this paper, we immunized BalBc mice with either the Hib vaccine or the Hib vaccine that adsorbs aluminum adjuvants (Hib-Al). Here, we analyzed the anti-PRP antibody level and immune response of different cells using cell and cytokine levels. We found that the Hib vaccine could induce a humoral and cellular immune response, and the Hib-Al vaccine could induce greater quantities of IFN-γ, IL-4, and IL-6 and more antigen-specific antibodies through B cells, Th1, Th2, and ILC3s in the spleen. Together, our findings demonstrate the serologic responses and immune response in terms of cell and cytokine levels induced by the Hib vaccine, and they also imply that the addition of aluminum hydroxide adjuvant could enhance the function of the Hib vaccine, which preliminarily reveals the mechanism of immune response induced by the Hib-related vaccine.
format Online
Article
Text
id pubmed-10594484
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105944842023-10-25 Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice He, Yao Zhao, Yuxiu Liang, Hongyang Wang, Xue Lan, Haoyue Tian, Dongyang Li, Yan Wang, Hui Diseases Article The Haemophilus influenzae type B (Hib) conjugate vaccine is the most effective way to prevent Hib infection in infants and young children, and it is designed to induce the production of antibodies against polyribosylribitol phosphate (PRP) to protect babies from infection. However, the mechanism of immunity induced by the Hib vaccine is not fully understood. Recently, with the development of the combination diphtheria and tetanus toxoids and acellular pertussis vaccines (DTaP), increasing numbers of manufacturers have begun to develop DTaP-based combination vaccines, like the combination vaccine diphtheria and tetanus toxoids and acellular pertussis and Hib conjugate vaccine (DTaP-Hib), which contains adjuvants. However, the Hib vaccine does not contain adjuvants. It was theorized that the Hib antigen has poor compatibility with aluminum adjuvants for unclear reasons. Therefore, understanding the mechanism of the Hib-vaccine-induced immune response and the influence of adjuvants on the Hib vaccine is of great significance. In this paper, we immunized BalBc mice with either the Hib vaccine or the Hib vaccine that adsorbs aluminum adjuvants (Hib-Al). Here, we analyzed the anti-PRP antibody level and immune response of different cells using cell and cytokine levels. We found that the Hib vaccine could induce a humoral and cellular immune response, and the Hib-Al vaccine could induce greater quantities of IFN-γ, IL-4, and IL-6 and more antigen-specific antibodies through B cells, Th1, Th2, and ILC3s in the spleen. Together, our findings demonstrate the serologic responses and immune response in terms of cell and cytokine levels induced by the Hib vaccine, and they also imply that the addition of aluminum hydroxide adjuvant could enhance the function of the Hib vaccine, which preliminarily reveals the mechanism of immune response induced by the Hib-related vaccine. MDPI 2023-10-13 /pmc/articles/PMC10594484/ /pubmed/37873783 http://dx.doi.org/10.3390/diseases11040139 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Yao
Zhao, Yuxiu
Liang, Hongyang
Wang, Xue
Lan, Haoyue
Tian, Dongyang
Li, Yan
Wang, Hui
Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice
title Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice
title_full Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice
title_fullStr Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice
title_full_unstemmed Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice
title_short Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice
title_sort systematic evaluation of the distribution of immune cells following subcutaneous administration of haemophilus influenzae type b vaccine to mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594484/
https://www.ncbi.nlm.nih.gov/pubmed/37873783
http://dx.doi.org/10.3390/diseases11040139
work_keys_str_mv AT heyao systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT zhaoyuxiu systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT lianghongyang systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT wangxue systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT lanhaoyue systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT tiandongyang systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT liyan systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice
AT wanghui systematicevaluationofthedistributionofimmunecellsfollowingsubcutaneousadministrationofhaemophilusinfluenzaetypebvaccinetomice