Cargando…
Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines
Antibodies and other new antibody-like formats have emerged as one of the most rapidly growing classes of biotherapeutic proteins. Understanding the structural features that drive antibody function and, consequently, their molecular recognition is critical for engineering antibodies. Here, we presen...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594513/ https://www.ncbi.nlm.nih.gov/pubmed/37873864 http://dx.doi.org/10.3390/antib12040067 |
_version_ | 1785124667778924544 |
---|---|
author | Fernández-Quintero, Monica L. Pomarici, Nancy D. Fischer, Anna-Lena M. Hoerschinger, Valentin J. Kroell, Katharina B. Riccabona, Jakob R. Kamenik, Anna S. Loeffler, Johannes R. Ferguson, James A. Perrett, Hailee R. Liedl, Klaus R. Han, Julianna Ward, Andrew B. |
author_facet | Fernández-Quintero, Monica L. Pomarici, Nancy D. Fischer, Anna-Lena M. Hoerschinger, Valentin J. Kroell, Katharina B. Riccabona, Jakob R. Kamenik, Anna S. Loeffler, Johannes R. Ferguson, James A. Perrett, Hailee R. Liedl, Klaus R. Han, Julianna Ward, Andrew B. |
author_sort | Fernández-Quintero, Monica L. |
collection | PubMed |
description | Antibodies and other new antibody-like formats have emerged as one of the most rapidly growing classes of biotherapeutic proteins. Understanding the structural features that drive antibody function and, consequently, their molecular recognition is critical for engineering antibodies. Here, we present the structural architecture of conventional IgG antibodies alongside other formats. We emphasize the importance of considering antibodies as conformational ensembles in solution instead of focusing on single-static structures because their functions and properties are strongly governed by their dynamic nature. Thus, in this review, we provide an overview of the unique structural and dynamic characteristics of antibodies with respect to their antigen recognition, biophysical properties, and effector functions. We highlight the numerous technical advances in antibody structure prediction and design, enabled by the vast number of experimentally determined high-quality structures recorded with cryo-EM, NMR, and X-ray crystallography. Lastly, we assess antibody and vaccine design strategies in the context of structure and dynamics. |
format | Online Article Text |
id | pubmed-10594513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105945132023-10-25 Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines Fernández-Quintero, Monica L. Pomarici, Nancy D. Fischer, Anna-Lena M. Hoerschinger, Valentin J. Kroell, Katharina B. Riccabona, Jakob R. Kamenik, Anna S. Loeffler, Johannes R. Ferguson, James A. Perrett, Hailee R. Liedl, Klaus R. Han, Julianna Ward, Andrew B. Antibodies (Basel) Review Antibodies and other new antibody-like formats have emerged as one of the most rapidly growing classes of biotherapeutic proteins. Understanding the structural features that drive antibody function and, consequently, their molecular recognition is critical for engineering antibodies. Here, we present the structural architecture of conventional IgG antibodies alongside other formats. We emphasize the importance of considering antibodies as conformational ensembles in solution instead of focusing on single-static structures because their functions and properties are strongly governed by their dynamic nature. Thus, in this review, we provide an overview of the unique structural and dynamic characteristics of antibodies with respect to their antigen recognition, biophysical properties, and effector functions. We highlight the numerous technical advances in antibody structure prediction and design, enabled by the vast number of experimentally determined high-quality structures recorded with cryo-EM, NMR, and X-ray crystallography. Lastly, we assess antibody and vaccine design strategies in the context of structure and dynamics. MDPI 2023-10-18 /pmc/articles/PMC10594513/ /pubmed/37873864 http://dx.doi.org/10.3390/antib12040067 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Fernández-Quintero, Monica L. Pomarici, Nancy D. Fischer, Anna-Lena M. Hoerschinger, Valentin J. Kroell, Katharina B. Riccabona, Jakob R. Kamenik, Anna S. Loeffler, Johannes R. Ferguson, James A. Perrett, Hailee R. Liedl, Klaus R. Han, Julianna Ward, Andrew B. Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines |
title | Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines |
title_full | Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines |
title_fullStr | Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines |
title_full_unstemmed | Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines |
title_short | Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines |
title_sort | structure and dynamics guiding design of antibody therapeutics and vaccines |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594513/ https://www.ncbi.nlm.nih.gov/pubmed/37873864 http://dx.doi.org/10.3390/antib12040067 |
work_keys_str_mv | AT fernandezquinteromonical structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT pomaricinancyd structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT fischerannalenam structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT hoerschingervalentinj structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT kroellkatharinab structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT riccabonajakobr structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT kamenikannas structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT loefflerjohannesr structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT fergusonjamesa structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT perretthaileer structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT liedlklausr structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT hanjulianna structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines AT wardandrewb structureanddynamicsguidingdesignofantibodytherapeuticsandvaccines |