Cargando…
An optimized protocol to detect ubiquitination modification of exogenous or endogenous proteins
Ubiquitination modification is an important post-translational modification that regulates the stability and function of proteins. Here, we present a protocol to detect the K27-linked polyubiquitination of exogenous and endogenous mitochondrial antiviral signaling protein. We describe steps for dete...
Autores principales: | Li, Xinyu, Jiang, Wei, Fang, Min |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594630/ https://www.ncbi.nlm.nih.gov/pubmed/37858474 http://dx.doi.org/10.1016/j.xpro.2023.102650 |
Ejemplares similares
-
An optimized protocol to detect protein ubiquitination and activation by ubiquitination assay in vivo and CCK-8 assay
por: Lin, Xiao-Tong, et al.
Publicado: (2023) -
Optimized protocols for chromatin immunoprecipitation of exogenously expressed epitope-tagged proteins
por: Fang, Wentong, et al.
Publicado: (2023) -
Endogenous and exogenous sex steroid hormones in asthma and allergy in females: protocol for a systematic review and meta-analysis
por: Nwaru, Bright I, et al.
Publicado: (2016) -
An optimized co-immunoprecipitation protocol for the analysis of endogenous protein-protein interactions in cell lines using mass spectrometry
por: Lagundžin, Dragana, et al.
Publicado: (2022) -
Studying miRNA–mRNA Interactions: An Optimized CLIP-Protocol for Endogenous Ago2-Protein
por: Stebel, Sophie, et al.
Publicado: (2022)