IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolution

Besides canonical microRNAs (miRNAs), sequence-based variations called isomiRs have biological relevance and diagnostic potential; however, accurate calling of these post-transcriptional modifications is challenging, especially for low input samples. Here, we present IsoSeek, a sequencing protocol t...

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Detalles Bibliográficos
Autores principales: van Eijndhoven, Monique A.J., Scheepbouwer, Chantal, Aparicio-Puerta, Ernesto, Hackenberg, Michael, Pegtel, D. Michiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594637/
https://www.ncbi.nlm.nih.gov/pubmed/37858475
http://dx.doi.org/10.1016/j.xpro.2023.102645

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