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CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces
[Image: see text] Streptomyces, a genus of Gram-positive bacteria, is known as nature’s medicine maker, producing a plethora of natural products that have huge benefits for human health, agriculture, and biotechnology. To take full advantage of this treasure trove of bioactive molecules, better gene...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594651/ https://www.ncbi.nlm.nih.gov/pubmed/37801665 http://dx.doi.org/10.1021/acssynbio.3c00464 |
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author | Bai, Chaoxian van Wezel, Gilles P. |
author_facet | Bai, Chaoxian van Wezel, Gilles P. |
author_sort | Bai, Chaoxian |
collection | PubMed |
description | [Image: see text] Streptomyces, a genus of Gram-positive bacteria, is known as nature’s medicine maker, producing a plethora of natural products that have huge benefits for human health, agriculture, and biotechnology. To take full advantage of this treasure trove of bioactive molecules, better genetic tools are required for the genetic engineering and synthetic biology of Streptomyces. We therefore developed CUBIC, a novel CUmate-Based Inducible CRISPR interference (CRISPRi) system that allows highly efficient and inducible gene knockdown in Streptomyces. Its broad application is shown by the specific and nondisruptive knockdown of genes involved in growth, development and antibiotic production in various Streptomyces species. To facilitate hyper-efficient plasmid construction, we adapted the Golden Gate assembly to achieve 100% cloning efficiency of the protospacers. We expect that the versatile plug-and-play CUBIC system will create new opportunities for research and innovation in the field of Streptomyces. |
format | Online Article Text |
id | pubmed-10594651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105946512023-10-25 CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces Bai, Chaoxian van Wezel, Gilles P. ACS Synth Biol [Image: see text] Streptomyces, a genus of Gram-positive bacteria, is known as nature’s medicine maker, producing a plethora of natural products that have huge benefits for human health, agriculture, and biotechnology. To take full advantage of this treasure trove of bioactive molecules, better genetic tools are required for the genetic engineering and synthetic biology of Streptomyces. We therefore developed CUBIC, a novel CUmate-Based Inducible CRISPR interference (CRISPRi) system that allows highly efficient and inducible gene knockdown in Streptomyces. Its broad application is shown by the specific and nondisruptive knockdown of genes involved in growth, development and antibiotic production in various Streptomyces species. To facilitate hyper-efficient plasmid construction, we adapted the Golden Gate assembly to achieve 100% cloning efficiency of the protospacers. We expect that the versatile plug-and-play CUBIC system will create new opportunities for research and innovation in the field of Streptomyces. American Chemical Society 2023-10-06 /pmc/articles/PMC10594651/ /pubmed/37801665 http://dx.doi.org/10.1021/acssynbio.3c00464 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Bai, Chaoxian van Wezel, Gilles P. CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces |
title | CUBIC: A Versatile Cumate-Based Inducible CRISPRi
System in Streptomyces |
title_full | CUBIC: A Versatile Cumate-Based Inducible CRISPRi
System in Streptomyces |
title_fullStr | CUBIC: A Versatile Cumate-Based Inducible CRISPRi
System in Streptomyces |
title_full_unstemmed | CUBIC: A Versatile Cumate-Based Inducible CRISPRi
System in Streptomyces |
title_short | CUBIC: A Versatile Cumate-Based Inducible CRISPRi
System in Streptomyces |
title_sort | cubic: a versatile cumate-based inducible crispri
system in streptomyces |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594651/ https://www.ncbi.nlm.nih.gov/pubmed/37801665 http://dx.doi.org/10.1021/acssynbio.3c00464 |
work_keys_str_mv | AT baichaoxian cubicaversatilecumatebasedinduciblecrisprisysteminstreptomyces AT vanwezelgillesp cubicaversatilecumatebasedinduciblecrisprisysteminstreptomyces |