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Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
OBJECTIVE: Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. METHODS: C57BL/6 mice were div...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594717/ https://www.ncbi.nlm.nih.gov/pubmed/37875866 http://dx.doi.org/10.1186/s12953-023-00220-x |
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author | Xu, Jia Liu, Jiacheng Qu, Yibai Jiang, Linhui Liang, Rongxin Li, Bohai Li, Lei Jiang, Yong |
author_facet | Xu, Jia Liu, Jiacheng Qu, Yibai Jiang, Linhui Liang, Rongxin Li, Bohai Li, Lei Jiang, Yong |
author_sort | Xu, Jia |
collection | PubMed |
description | OBJECTIVE: Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. METHODS: C57BL/6 mice were divided into three groups and given ordinary drinking water, ordinary drinking water plus a saline osmotic pump, or drinking water containing β-aminopropionitrile (BAPN) (1 g/kg/d) plus an angiotensin II (Ang II) (1 μg/kg/min) osmotic pump. Haematoxylin and eosin staining of thoracic aortic tissues was performed. The basic characteristics of exosomes were analysed. Differentially expressed proteins (DEPs) were identified by LC‒MS/MS. Protein‒protein networks and enrichment analysis were used to explore possible molecular mechanisms. RESULTS: The present study elucidated the protein expression profile of serum exosomes in mice with TAA induced by BAPN combined with Ang II. In this work, the expression of a total of 196 proteins was significantly dysregulated in serum exosomes of mice with TAA, with 122 proteins significantly upregulated and 74 proteins markedly downregulated. Notably, Haptoglobin (Hp) and Serum amyloid p-component (Sap) identified based on the PPI network were significantly upregulated and have been strongly linked to cardiovascular disease. Interestingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the upregulated and downregulated proteins were involved in the complement and coagulation cascade pathways. CONCLUSIONS: This study showed that the identified DEPs have potential as biomarkers for the diagnosis of TAA and provided a more comprehensive understanding of the pathophysiological mechanisms of TAA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12953-023-00220-x. |
format | Online Article Text |
id | pubmed-10594717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105947172023-10-25 Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm Xu, Jia Liu, Jiacheng Qu, Yibai Jiang, Linhui Liang, Rongxin Li, Bohai Li, Lei Jiang, Yong Proteome Sci Research OBJECTIVE: Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. METHODS: C57BL/6 mice were divided into three groups and given ordinary drinking water, ordinary drinking water plus a saline osmotic pump, or drinking water containing β-aminopropionitrile (BAPN) (1 g/kg/d) plus an angiotensin II (Ang II) (1 μg/kg/min) osmotic pump. Haematoxylin and eosin staining of thoracic aortic tissues was performed. The basic characteristics of exosomes were analysed. Differentially expressed proteins (DEPs) were identified by LC‒MS/MS. Protein‒protein networks and enrichment analysis were used to explore possible molecular mechanisms. RESULTS: The present study elucidated the protein expression profile of serum exosomes in mice with TAA induced by BAPN combined with Ang II. In this work, the expression of a total of 196 proteins was significantly dysregulated in serum exosomes of mice with TAA, with 122 proteins significantly upregulated and 74 proteins markedly downregulated. Notably, Haptoglobin (Hp) and Serum amyloid p-component (Sap) identified based on the PPI network were significantly upregulated and have been strongly linked to cardiovascular disease. Interestingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the upregulated and downregulated proteins were involved in the complement and coagulation cascade pathways. CONCLUSIONS: This study showed that the identified DEPs have potential as biomarkers for the diagnosis of TAA and provided a more comprehensive understanding of the pathophysiological mechanisms of TAA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12953-023-00220-x. BioMed Central 2023-10-24 /pmc/articles/PMC10594717/ /pubmed/37875866 http://dx.doi.org/10.1186/s12953-023-00220-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Xu, Jia Liu, Jiacheng Qu, Yibai Jiang, Linhui Liang, Rongxin Li, Bohai Li, Lei Jiang, Yong Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
title | Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
title_full | Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
title_fullStr | Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
title_full_unstemmed | Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
title_short | Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
title_sort | label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594717/ https://www.ncbi.nlm.nih.gov/pubmed/37875866 http://dx.doi.org/10.1186/s12953-023-00220-x |
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