Cargando…

Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm

OBJECTIVE: Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. METHODS: C57BL/6 mice were div...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Jia, Liu, Jiacheng, Qu, Yibai, Jiang, Linhui, Liang, Rongxin, Li, Bohai, Li, Lei, Jiang, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594717/
https://www.ncbi.nlm.nih.gov/pubmed/37875866
http://dx.doi.org/10.1186/s12953-023-00220-x
_version_ 1785124707526246400
author Xu, Jia
Liu, Jiacheng
Qu, Yibai
Jiang, Linhui
Liang, Rongxin
Li, Bohai
Li, Lei
Jiang, Yong
author_facet Xu, Jia
Liu, Jiacheng
Qu, Yibai
Jiang, Linhui
Liang, Rongxin
Li, Bohai
Li, Lei
Jiang, Yong
author_sort Xu, Jia
collection PubMed
description OBJECTIVE: Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. METHODS: C57BL/6 mice were divided into three groups and given ordinary drinking water, ordinary drinking water plus a saline osmotic pump, or drinking water containing β-aminopropionitrile (BAPN) (1 g/kg/d) plus an angiotensin II (Ang II) (1 μg/kg/min) osmotic pump. Haematoxylin and eosin staining of thoracic aortic tissues was performed. The basic characteristics of exosomes were analysed. Differentially expressed proteins (DEPs) were identified by LC‒MS/MS. Protein‒protein networks and enrichment analysis were used to explore possible molecular mechanisms. RESULTS: The present study elucidated the protein expression profile of serum exosomes in mice with TAA induced by BAPN combined with Ang II. In this work, the expression of a total of 196 proteins was significantly dysregulated in serum exosomes of mice with TAA, with 122 proteins significantly upregulated and 74 proteins markedly downregulated. Notably, Haptoglobin (Hp) and Serum amyloid p-component (Sap) identified based on the PPI network were significantly upregulated and have been strongly linked to cardiovascular disease. Interestingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the upregulated and downregulated proteins were involved in the complement and coagulation cascade pathways. CONCLUSIONS: This study showed that the identified DEPs have potential as biomarkers for the diagnosis of TAA and provided a more comprehensive understanding of the pathophysiological mechanisms of TAA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12953-023-00220-x.
format Online
Article
Text
id pubmed-10594717
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-105947172023-10-25 Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm Xu, Jia Liu, Jiacheng Qu, Yibai Jiang, Linhui Liang, Rongxin Li, Bohai Li, Lei Jiang, Yong Proteome Sci Research OBJECTIVE: Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. METHODS: C57BL/6 mice were divided into three groups and given ordinary drinking water, ordinary drinking water plus a saline osmotic pump, or drinking water containing β-aminopropionitrile (BAPN) (1 g/kg/d) plus an angiotensin II (Ang II) (1 μg/kg/min) osmotic pump. Haematoxylin and eosin staining of thoracic aortic tissues was performed. The basic characteristics of exosomes were analysed. Differentially expressed proteins (DEPs) were identified by LC‒MS/MS. Protein‒protein networks and enrichment analysis were used to explore possible molecular mechanisms. RESULTS: The present study elucidated the protein expression profile of serum exosomes in mice with TAA induced by BAPN combined with Ang II. In this work, the expression of a total of 196 proteins was significantly dysregulated in serum exosomes of mice with TAA, with 122 proteins significantly upregulated and 74 proteins markedly downregulated. Notably, Haptoglobin (Hp) and Serum amyloid p-component (Sap) identified based on the PPI network were significantly upregulated and have been strongly linked to cardiovascular disease. Interestingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the upregulated and downregulated proteins were involved in the complement and coagulation cascade pathways. CONCLUSIONS: This study showed that the identified DEPs have potential as biomarkers for the diagnosis of TAA and provided a more comprehensive understanding of the pathophysiological mechanisms of TAA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12953-023-00220-x. BioMed Central 2023-10-24 /pmc/articles/PMC10594717/ /pubmed/37875866 http://dx.doi.org/10.1186/s12953-023-00220-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Xu, Jia
Liu, Jiacheng
Qu, Yibai
Jiang, Linhui
Liang, Rongxin
Li, Bohai
Li, Lei
Jiang, Yong
Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
title Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
title_full Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
title_fullStr Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
title_full_unstemmed Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
title_short Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
title_sort label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594717/
https://www.ncbi.nlm.nih.gov/pubmed/37875866
http://dx.doi.org/10.1186/s12953-023-00220-x
work_keys_str_mv AT xujia labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT liujiacheng labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT quyibai labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT jianglinhui labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT liangrongxin labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT libohai labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT lilei labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm
AT jiangyong labelfreequantitativeproteomicanalysisofserumexosomesinmicewiththoracicaorticaneurysm