Cargando…

Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus

BACKGROUND: Given the rising occurrence of antibiotic resistance due to the existence and ongoing development of resistant bacteria and phenotypes, the identification of new treatments and sources of antimicrobial agents is of utmost urgency. An important strategy for tackling bacterial resistance i...

Descripción completa

Detalles Bibliográficos
Autores principales: de Jesus, Genilson Silva, Silva Trentin, Danielle, Barros, Thayná Fernandes, Ferreira, Alda Maria Teixeira, de Barros, Bruna Castro, de Oliveira Figueiredo, Patrícia, Garcez, Fernanda Rodrigues, dos Santos, Érica Luiz, Micheletti, Ana Camila, Yoshida, Nidia Cristiane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594757/
https://www.ncbi.nlm.nih.gov/pubmed/37872494
http://dx.doi.org/10.1186/s12906-023-04147-w
_version_ 1785124717073530880
author de Jesus, Genilson Silva
Silva Trentin, Danielle
Barros, Thayná Fernandes
Ferreira, Alda Maria Teixeira
de Barros, Bruna Castro
de Oliveira Figueiredo, Patrícia
Garcez, Fernanda Rodrigues
dos Santos, Érica Luiz
Micheletti, Ana Camila
Yoshida, Nidia Cristiane
author_facet de Jesus, Genilson Silva
Silva Trentin, Danielle
Barros, Thayná Fernandes
Ferreira, Alda Maria Teixeira
de Barros, Bruna Castro
de Oliveira Figueiredo, Patrícia
Garcez, Fernanda Rodrigues
dos Santos, Érica Luiz
Micheletti, Ana Camila
Yoshida, Nidia Cristiane
author_sort de Jesus, Genilson Silva
collection PubMed
description BACKGROUND: Given the rising occurrence of antibiotic resistance due to the existence and ongoing development of resistant bacteria and phenotypes, the identification of new treatments and sources of antimicrobial agents is of utmost urgency. An important strategy for tackling bacterial resistance involves the utilization of drug combinations, and natural products derived from plants hold significant potential as a rich source of bioactive compounds that can act as effective adjuvants. This study, therefore, aimed to assess the antibacterial potential and the chemical composition of Miconia albicans, a Brazilian medicinal plant used to treat various diseases. METHODS: Ethanolic extracts from leaves and stems of M. albicans were obtained and subsequently partitioned to give the corresponding hexane, chloroform, ethyl acetate, and hydromethanolic phases. All extracts and phases had their chemical constitution investigated by HPLC–DAD-MS/MS and GC–MS and were assessed for their antibiofilm and antimicrobial efficacy against Staphylococcus aureus. Furthermore, their individual effects and synergistic potential in combination with antibiotics were examined against clinical strains of both S. aureus and Acinetobacter baumannii. In addition, 10 isolated compounds were obtained from the leaves phases and used for confirmation of the chemical profiles and for antibacterial assays. RESULTS: Based on the chemical profile analysis, 32 compounds were successfully or tentatively identified, including gallic and ellagic acid derivatives, flavonol glycosides, triterpenes and pheophorbides. Extracts and phases obtained from the medicinal plant M. albicans demonstrated synergistic effects when combined with the commercial antibiotics ampicillin and ciprofloxacin, against multi-drug resistant bacteria S. aureus and A. baumannii, restoring their antibacterial efficacy. Extracts and phases also exhibited antibiofilm property against S. aureus. Three key compounds commonly found in the samples, namely gallic acid, quercitrin, and corosolic acid, did not exhibit significant antibacterial activity when assessed individually or in combination with antibiotics against clinical bacterial strains. CONCLUSIONS: Our findings reveal that M. albicans exhibits remarkable adjuvant potential for enhancing the effectiveness of antimicrobial drugs against resistant bacteria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-023-04147-w.
format Online
Article
Text
id pubmed-10594757
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-105947572023-10-25 Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus de Jesus, Genilson Silva Silva Trentin, Danielle Barros, Thayná Fernandes Ferreira, Alda Maria Teixeira de Barros, Bruna Castro de Oliveira Figueiredo, Patrícia Garcez, Fernanda Rodrigues dos Santos, Érica Luiz Micheletti, Ana Camila Yoshida, Nidia Cristiane BMC Complement Med Ther Research BACKGROUND: Given the rising occurrence of antibiotic resistance due to the existence and ongoing development of resistant bacteria and phenotypes, the identification of new treatments and sources of antimicrobial agents is of utmost urgency. An important strategy for tackling bacterial resistance involves the utilization of drug combinations, and natural products derived from plants hold significant potential as a rich source of bioactive compounds that can act as effective adjuvants. This study, therefore, aimed to assess the antibacterial potential and the chemical composition of Miconia albicans, a Brazilian medicinal plant used to treat various diseases. METHODS: Ethanolic extracts from leaves and stems of M. albicans were obtained and subsequently partitioned to give the corresponding hexane, chloroform, ethyl acetate, and hydromethanolic phases. All extracts and phases had their chemical constitution investigated by HPLC–DAD-MS/MS and GC–MS and were assessed for their antibiofilm and antimicrobial efficacy against Staphylococcus aureus. Furthermore, their individual effects and synergistic potential in combination with antibiotics were examined against clinical strains of both S. aureus and Acinetobacter baumannii. In addition, 10 isolated compounds were obtained from the leaves phases and used for confirmation of the chemical profiles and for antibacterial assays. RESULTS: Based on the chemical profile analysis, 32 compounds were successfully or tentatively identified, including gallic and ellagic acid derivatives, flavonol glycosides, triterpenes and pheophorbides. Extracts and phases obtained from the medicinal plant M. albicans demonstrated synergistic effects when combined with the commercial antibiotics ampicillin and ciprofloxacin, against multi-drug resistant bacteria S. aureus and A. baumannii, restoring their antibacterial efficacy. Extracts and phases also exhibited antibiofilm property against S. aureus. Three key compounds commonly found in the samples, namely gallic acid, quercitrin, and corosolic acid, did not exhibit significant antibacterial activity when assessed individually or in combination with antibiotics against clinical bacterial strains. CONCLUSIONS: Our findings reveal that M. albicans exhibits remarkable adjuvant potential for enhancing the effectiveness of antimicrobial drugs against resistant bacteria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-023-04147-w. BioMed Central 2023-10-23 /pmc/articles/PMC10594757/ /pubmed/37872494 http://dx.doi.org/10.1186/s12906-023-04147-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
de Jesus, Genilson Silva
Silva Trentin, Danielle
Barros, Thayná Fernandes
Ferreira, Alda Maria Teixeira
de Barros, Bruna Castro
de Oliveira Figueiredo, Patrícia
Garcez, Fernanda Rodrigues
dos Santos, Érica Luiz
Micheletti, Ana Camila
Yoshida, Nidia Cristiane
Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus
title Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus
title_full Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus
title_fullStr Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus
title_full_unstemmed Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus
title_short Medicinal plant Miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant Acinetobacter baumannii and Staphylococcus aureus
title_sort medicinal plant miconia albicans synergizes with ampicillin and ciprofloxacin against multi-drug resistant acinetobacter baumannii and staphylococcus aureus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594757/
https://www.ncbi.nlm.nih.gov/pubmed/37872494
http://dx.doi.org/10.1186/s12906-023-04147-w
work_keys_str_mv AT dejesusgenilsonsilva medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT silvatrentindanielle medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT barrosthaynafernandes medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT ferreiraaldamariateixeira medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT debarrosbrunacastro medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT deoliveirafigueiredopatricia medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT garcezfernandarodrigues medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT dossantosericaluiz medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT michelettianacamila medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus
AT yoshidanidiacristiane medicinalplantmiconiaalbicanssynergizeswithampicillinandciprofloxacinagainstmultidrugresistantacinetobacterbaumanniiandstaphylococcusaureus