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One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions

[Image: see text] Fe–S clusters are essential cofactors mediating electron transfer in respiratory and metabolic networks. However, obtaining active [4Fe-4S] proteins with heterologous expression is challenging due to (i) the requirements for [4Fe-4S] cluster assembly, (ii) the O(2) lability of [4Fe...

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Detalles Bibliográficos
Autores principales:	Wang, Po-Hsiang, Nishikawa, Shota, McGlynn, Shawn Erin, Fujishima, Kosuke
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594875/ https://www.ncbi.nlm.nih.gov/pubmed/37467114 http://dx.doi.org/10.1021/acssynbio.3c00155

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